隐丹参酮对荷瘤小鼠化疗后免疫功能低下的影响

目的探讨隐丹参酮（CPT）对荷瘤小鼠顺铂（DDP）化疗后免疫功能低下的影响。方法40X小鼠分为5组，每组8只，Pc9细胞建移植瘤动物模型，分别为空白对照组组、模型组、CPT组（100mg／kg）、DDP组（2mg／kg）及联合用药（100mgCPT＋2mg／kgDDP）组，隐丹参酮连续给药15d，顺铂2次／周给药（每周第1天、第4天），停药24h后，测脾脏指数、CCK8检测T细胞增殖率、流式细胞术检测T细胞亚群、ELISA法检测荷瘤小鼠血清中IFN-1、IL-2的含量。结果与空白对照组比较，模型组免疫功能受到明显抑制（P〈O．01）；与模型组比较，CPT可明显提高荷瘤小鼠脾脏指数、T细胞增殖率、CD3^＋、CD8^＋、NK细胞、IFN-1、IL-2的表达（P〈001），而DDP的作用相反（P〈O01或P〈O．05）；与顺铂组比较，CPT组、联合用药组脾脏指数、T细胞增殖率、CD3^＋、NK细胞、IFN-γ、IL-2表达明显上升（P〈001），而CD4^＋、CD8^＋、CD4^＋／CD8^＋变化不明显（P〉005）；与隐丹参酮组比较，联合用药组脾脏指数、T细胞亚群、IL-2等差异无统计学意义（P〉0．05），T细胞增殖数量、IFN-γ差异有统计学意义（P〈O．01或P〈0．05）。结论隐丹参酮可提高化疗后机体的免疫功能，其机制可能与隐丹参酮提高血清中IFN-1、IL-2含量相关，对机体的免疫脏器也有-定的保护作用，具有-定的临床应用前景。

Objective To study the immunodeficiency of cryptotanshinone （ CPT ） on post-chemotherapeutic tumor-bearing mice. Methods Lung adenocarcinoma PC9 cell transplanted tumor animal models were divided into five groups： control group, model group, CPT group （ 100mg/kg ） , DDP group （2mg/kg） , and the combination group of CPT and DDP （ 100mg CPT＋2mg/kg DDP ） , each group had eight mice. Cryptotanshinone was administered continuously to 15d, cisplatin 2 times / week （first days, fourth days per week ） . After the withdrawal of 24h, the spleen index, the proliferation rate of T cells were measured by CCKS, the T cell subgroup was detected by flow cytometry, and the content of IFN- gamma and IL-2 in the serum of the tumor bearing mice was detected by ELISA. Results Compared with the blank control group, the immune function of the model group was significantly inhibited （P〈0.01） . Compared with the model group, the spleen index, T cell proliferation rate, CD3＋, CD8＋, NK cells, IFN- gamma and IL-2 expression were significantly increased by CPT in the tumor bearing mice （ P〈0.01 ） , but the effect of DDP was opposite （P〈0.01 or P〈0.05 ） . Compared with cisplatin group, the expression of spleen index, T cell proliferation rate, CD3＋, NK cell, IFN- gamma and IL-2 in CPT group and combined drug group increased significantly （ P〈0.01 ） , while CD4＋, CD8＋ and CD4＋/CD8＋ were not significant （P〉0.05） . Compared with the cryptotanshinone group, there was no significant difference in the spleen index, T cell subgroup and IL-2 （P〉0.05） in the combination group （P〉0.05） . The number of T cells and the difference of IFN- gamma were statistically significant （P〈0.01 or P〈0.05 ） . Conclusion CPT improves immune function after chemotherapy, and its mechanism may be related to the increase of the content of IFN- gamma and IL-2 in serum by cryptotanshinone. It also has a certain protective effect on the immune organs of the body, and has a certain clinical application prospect.