十字花科蔬菜摄入量及尿液中异硫氰酸盐水平与男性肺癌关系的巢式病例-对照研究

A nested case-control study of cruciferous vegetables intake, urinary isothiocyanates level and lung cancer risk among men in urban Shanghai

目的 探讨饮食十字花科蔬菜（CV）摄入、尿液中异硫氰酸盐（ITC）水平与男性肺癌发病风险的关系。方法 在上海男性健康队列的基础上开展巢式病例-对照研究，选择随访时间2010年12月31日之前经确诊的新发肺癌病例443例，利用密度抽样法按1∶2的比例抽取对照885名。采用食物频率问卷调查饮食CV的摄入情况，使用高效液相色谱法测定对象尿液中的ITC代谢量。按照对照组的CV摄入量、尿液ITC代谢量进行四分位数分组，以最低组为参照组，采用条件Logistic回归模型分析CV摄入、尿中ITC代谢水平和男性肺癌的关系。结果 病例组、对照组CV摄入量的M（P25，P75）分别为80.05（46.89，129.04）、97.68（55.25，151.72）g/d（Z=-3.93，P〈0.001）;尿液ITC代谢量的M（P25，P75）分别为1.256（0.474，3.836）、1.244（0.484，3.004）μmol/g肌酐（Z=-0.39，P=0.697）。调整年龄、教育程度、吸烟、饮酒等混杂因素后，与尿液中ITC水平最低组（〈0.484 μmol/g肌酐）相比，最高组（≥3.004 μmol/g肌酐）肺癌的OR（95%CI）值为1.25（0.87~1.80）；与十字花科蔬菜摄入水平最低组（〈55.25 g/d）相比，最高组（≥151.71 g/d）肺癌的OR（95%CI）值为0.66（0.43~1.02）。结论 未发现CV摄入量、尿液中ITC代谢水平与男性肺癌的发病风险差异有统计学意义。

Objective To investigate the association between consumption of cruciferous vegetables （CV）, level of urinary isothiocyanates （ITC） and the risk of lung cancer among man in urban Shanghai.Methods A nested case-control study was conducted within the Shanghai Men＇s Health Study. Using incidence density sampling with a 2∶1 control to case selection ratio, 885 controls were selected to match 443 lung cancer cases diagnosed prior December 31, 2010. A food-frequency questionnaire was administered to estimate CV consumption. The high performance liquid chromatography method was applied to measure urinary ITC level. The CV intake and urinary ITC level were divided into quartiles according to distribution of control group. The lowest quartile was as a reference group. Conditional logistic regression model was used to analyze the relationship between CV intake, urinary ITC level and the risk of lung cancer.Results The cruciferous vegetables intake median （P25, P75） in cases and controls were 80.05 （46.89, 129.04） and 97.68 （55.25, 151.72） g/d （Z=-3.93, P〈0.001）. The urinary ITC level were 1.256 （0.474, 3.836） and 1.244 （0.484, 3.004） μmol/g Cr （Z=-0.39, P=0.697）. After adjusting for potential confounding factors such as age, education level, smoking and alcohol consumption, for urinary ITC level, the OR（95%CI） for the highest quartile（≥3.004 μmol/g Cr） was 1.25 （0.87-1.80） compared with the lowest quartile（〈0.484 μmol/g）. For CV intake, the OR（95%CI） for the highest quartile（≥151.71 g/d） was 0.66 （0.43-1.02） compared with the lowest quartile（〈55.25 g/d）.Conclusion No association was found between the CV intake, urinary ITC level and lung cancer risk in men.