贮藏条件对红皮云杉种鳞多酚含量及抗氧化功能的影响

Effects of Storage Conditions on the Content and Antioxidant Activity of Polyphenols from Picea koraiensis Nakai Seed Scales

本试验以红皮云杉种鳞为原料,研究pH值、光照、温度、金属离子及添加剂等贮藏条件对红皮云杉种鳞多酚含量及抗氧化功能的影响。试验结果显示,4℃低温和避光条件下,红皮云杉种鳞多酚最稳定,保留率依次为93.08%、87.23%,DPPH自由基清除率依次为80.65%、82.16%,总还原力OD值依次为0.854、0.798。酸性条件对红皮云杉种鳞多酚含量影响较小,碱性条件破坏红皮云杉种鳞多酚,pH 2条件下多酚保留率、DPPH·清除率和总还原力OD值分别是pH 10的2.21倍、4.56倍、2.78倍。添加钠、钾、钙离子对红皮云杉种鳞多酚含量和抗氧化功能无显著影响,而铜、锌、铁离子能够显著降低多酚含量和抗氧化功能。浓度高于30%的蔗糖和0.01%苯甲酸钠能够延缓红皮云杉种鳞多酚的降解,对抗氧化稳定性无显著影响。本试验在降解试验的基础上探究贮藏条件对红皮云杉种鳞多酚抗氧化功能的影响,将为多酚的应用开创新的前景。

In this experiment, the effect of storage conditions, such as pH, light, temperature, metal ions and additives, on the content, anti-oxidative capacity and stability of polyphenol in the cone scale of Picea koraiensis Nakai were researched. The polyphenols in Picea koraiensis Nakai cone scale showed the highest stability with the content rates of 93.08% at 4℃ and relatively high stability in darkness with content rates of 87.23%. under these two conditions, the DPPH scavenging rates of phenol were 80.65% and 82.16%, respectively, and the total reducing capacity（OD value） of polyphenols in Picea koraiensis Nakai cone scales were 0.854 and 0.798, respectively. The effects of pH on these three parameters indicated that acidic conditions were more compatible than basic ones. The content rate, DPPH scavenging rate and total reducing capacity of the polyphenols at pH2 were 2.21, 4.56, and 2.78 times, respectively, than those at pH 10. Ions showed different effects depending on the kinds. The ions Na＋, K＋, and Ca2＋ showed slight effect, while Cu2＋, Zn2＋, and Fe3＋ had significant impacts on the polyphenols content and anti-oxidative ability. In terms of additives, sucrose solution with concentration higher than 30% and 0.01% sodium benzoate solution could retard the degradation of polyphenols in the cone scale, while their effects on the anti-oxidative ability were not significant. This research will provide a new prospect for the application of Picea koraiensis Nakai cone scale polyphenols.