樱桃仁的小鼠饲喂安全性评价

Evaluation of the Safety of Cherry Kernel Used as a Feed Ingredient

研究中国樱桃仁的饲用安全性,为樱桃仁的综合加工利用提供一定的参考依据。用添加不同量(10%,20%和30%)樱桃仁的饲料饲喂小鼠28 d,以普通饲料饲喂的小鼠为对照组,通过称重法分析小鼠的体重变化和脏器系数、用血细胞分析仪分析小鼠血液组分(白细胞数量、红细胞数量、红细胞压积、平均红细胞体积、血红蛋白浓度、平均血红蛋白浓度、平均血红蛋白含量、血小板计数、血小板压积、平均血小板体积、血小板分布宽度)、用化学分析法分析血液中谷丙转氨酶和谷草转氨酶活性以评价樱桃仁的饲用安全性。结果表明:在饲喂周期内,小鼠无异常症状和体征,除少数指标所测结果与对照组有所差别,大部分指标结果都不存在显著性差异,所有指标测得值均在小鼠正常生理指标范围内,未发现毒害作用,鉴于此,初步可以认为樱桃仁作为饲料添加是安全的。

Studying the feeding safety of Chinese cherry kernel could provide some reference for the comprehensive processing and utilization of cherry kernel. With the addition of different amounts（10%, 20% and 30%） of cherry kernel fed mice for 28 days, the mice fed with normal diet as the control group, body weight changes and organ coefficients of the mice were analyzed by weighing method, the blood components of the mice（the number of leucocytes, the number of red blood cells, backlog of red blood cells, average erythrocyte volume, hemoglobin concentration, the average hemoglobin concentration, average hemoglobin content, platelet count, thrombocytocrit, mean platelet volume, platelet distribution width） were analyzed by a hematology analyzer. The blood alanine aminotransferase and aspartate aminotransferase activity were analyzed by the chemical method to evaluate the feeding safety of cherry kernel. There were no abnormal symptoms and signs in mice during the feeding period. Most of the indicators did not show significant differences in the results except for a few indicators of the results compared with the control group, the measured indicators were within the normal physiological range of mice and no toxic effects were found. Accordingly, it can be preliminarily concluded that the mice fed the cherry kernel-containing feed were safe and non-toxic.