猕猴桃过氧化物酶基因在不同器官中的表达分析

Expression Analysis of Peroxidase Genes in Different Organs of Kiwifruit

为了解猕猴桃过氧化物酶基因的调控功能,试验采用q RT-PCR技术分析了Ad POD27和Ad POD64在猕猴桃不同器官中的表达模式。结果表明,在猕猴桃不同器官中,Ad POD27和Ad POD64的表达模式较为一致,均在根中大量表达,其次是茎,而在果实中表达量最低。此外,除果实以外,Ad POD27在猕猴桃不同器官中的表达水平均高于Ad POD64。研究认为,Ad POD27与Ad POD64可能参与了猕猴桃根和茎的木质化形成过程,且Ad POD27发挥的调控作用更为明显。

To investigate the regulatory functions of peroxidase （POD） genes in kiwifruit, the expression patterns of AdPOD27 and AdPOD64 in different organs were analyzed by using qRT-PCR. The results showed that the expression patterns of AdPOD27 and AdPOD64 in different organs of kiwifruit were similar, and all of them were high-expressed in roots, and low-expressed in fruits. In addition, the expression level of A dPOD27 was higher than that of A dPOD64 （except fruits） . Our studies suggested that, A dPOD27 and A dPOD64 would involve in lignification of roots and stems in kiwifruit, and A dPOD27 might play more important regulatory functions in the process..