微流控芯片上循环肿瘤细胞高效率分选研究

High-Efficiency Separation of Circulating Tumor Cell Using Microfluidic Chips

本研究将高通量的惯性微流技术与高回收率的空间位阻技术相结合,设计并制备了一种用于血液中循环肿瘤细胞高效率分选的被动式微流控芯片。该装置通过设置过滤区域、惯性聚集区域、惯性分离区域和空间位阻区域,实现了循环肿瘤细胞的高效率分选,可在不使用任何抗体标记的情况下,从2%血细胞比容血液样品中分选出人源性乳腺癌细胞和人源性宫颈癌细胞,分离速率均达到3.43×10~7 cell/min,癌细胞回收率与富集率均分别大于95.20%和2.05×10~5。对比目前存在的诸多循环肿瘤细胞分离方法,该技术具有高通量、高回收率及高富集率的优势,可为建立癌症患者微型诊断分析平台提供新的技术手段。

In this paper,a passive microfluidic device was developed for high-efficiency isolation of circulating tumor cell using a unique combination of steric hindrance and inertial microfluidics.The serial complementary combination of two different mechanism sorting methods achieved high-throughput and highly sensitive cell separation.The device comprised four functional regions：filter region,inertial focusing region,inertial separation region,and steric hindrance region.As an actual application of this microfluidic system,circulating tumor cells（MCF-7 and HeLa cells）spiked into 2% Hct blood were successfully separated with throughput of 3.43×10~7 cells/min,cell recovery of 95.20%,and an impressive cell enrichment of2.05×10~5-fold.Compared with existing circulating tumor cell isolation methods,the current microfluidic device possesses high throughput,cell recovery,and cell enrichment.It provides a new microfluidic technology for establishing micro-diagnosis platform for cancer patients.