12种真菌钾离子通道蛋白生物信息学分析

Bioinformatics Analysis of 12 Fungal Potassium Channel Proteins

利用生物信息学对真菌钾离子通道蛋白进行序列分析,推断并预测其结构和功能。本文筛选了酿酒酵母、黄曲霉、烟曲霉、禾谷镰刀菌、玉米黑粉菌、布拉克须霉等12种真菌的钾离子通道蛋白,综合采用Blast、Prot Param、Signal P和Swiss-Model等生物信息学网站和软件对这些蛋白的理化性质、系统进化、蛋白结构和功能域等进行了分析。结果表明:所选择的钾离子通道蛋白氨基酸序列长度在659~928 aa之间,理论等电点在5.45~9.54之间,主要定位在质膜、液泡上。二级结构分析表明,真菌钾离子通道蛋白均由α-螺旋、β-折叠、无规则卷曲和延伸链等结构元件组成,其空间结构高度相似,且12种钾离子通道蛋白都拥有保守的Tx GYGD钾离子选择器特征片段。

Bioinformatics was used to analyze the sequence of potassium channel proteins in fungi,so as to infer and predict its structure and function. This paper screened the saccharomy cescerevisiae,Aspergillus flavus,A. fumigatus,Fusarium graminearum,A.flavus,Phycomyces blakesleeanus 12 kinds of fungi of the potassium ion channel proteins,such as combination Blast,Prot Param,Signal P and Swiss-Model of bioinformatics website and software for the physical and chemical properties of these proteins,system evolution,the structure and function of protein domain were analyzed.The results showed that the amino acid sequence length of the selected potassium channel protein was between 659~928 aa,and the theoretical equivalence point was between 5.45~9.54,mainly located in the plasma membrane and vacuole. Secondary structure analysis showed that fungus potassium ion channel proteins were by alpha-helix,beta-folding,no rules curly and chain extension structure components,such as highly similar,its spatial structure and 12 kinds of potassium ion channel proteins had a conservative Tx GYGD potassium characteristic fragment ions selectors.