不同粒度刺梨果渣对高血脂小鼠血脂及组织抗氧化活性的影响

Effects of Different Sizes of Rosa roxbunghii Pomace on Blood Lipids and Tissue Antioxidant Activity in Hyperlipemia Mice

为研究不同粒度刺梨果渣对高血脂小鼠血脂水平及组织抗氧化活性的影响,本实验采用高脂饲料喂养小鼠建立高血脂模型,以60目和300目不同剂量刺梨果渣制备悬浮液分别灌胃喂养14 d,测定小鼠血清总胆固醇(TC)、甘油三酯(TG)和高密度脂蛋白(HDL-C)含量,以及心、肝、肾组织氧化水平相关值。结果表明,经14 d喂养后,与模型组相比,300目果渣组小鼠TC、TG含量极显著下降(p<0.01),高密度脂蛋白(HDL-C)含量显著上升(p<0.05),其中主要以高剂量组效果佳。同时,通过测定各组小鼠心、肝、肾氧化指标表明,两种果渣均能明显升高组织总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-PX)及超氧化物歧化酶(SOD)含量并降低丙二醛(MDA)水平(p<0.01)。结论:300目刺梨果渣可明显降低高血脂小鼠TC、TG并提高HDL-C水平,且两种粒度刺梨果渣粉均能较好改善氧化应激作用,有助于缓解高血脂症状。

To discover effects of different sizes of Rosa roxbunghii pomace on blood lipids level and tissue mice.Then the mice were respectively fed with the soliquoid of Rosa roxbunghii pomace through 300 mesh sieve and 60 mesh sieve by intragastric administration for 14 days. Subsequently,their serum total cholesterol（ TC）,triglyceride（ TG） and high density lipoprotein cholesterol（ HDL-C） content,and indexes about oxidation in heart,liver and kidney were determined. The result showed that after a fortnight＇s feed,TC and TG content of 300-mesh sieve pomace treated groups fell significantly（ p〈0.01） while HDL-C content rose markedly（ p〈0.01） compared to the model group,in which high-dose group had the best potence. Meanwhile,it indicated by the determination of oxidative indexes that both sizes of the pomace could significantly enhance the total antioxidant capacity（ T-AOC）,glutathione peroxidase（ GSH-PX） and superoxide dismutase（ SOD） content and lower the malondialdehyde（ MDA） level in heart,liver and kidney（ p〈0.01）. Therefore,the Rosa roxbunghii pomace through 300-mesh sieve had an impressive effect in reducing TC,TG and elevating HDL-C content in hyperlipemia mice.Besides,both sizes of the Rosa roxbunghii pomace could effectively improve the oxidative stress,which was good for relieving the symptoms of hypelipemia.