应用高通量技术分析F446饱水木漆器中微生物群落结构多样性

Microbial community structure and diversity in waterlogged wood and lacquer named F446 by Illumina Mi Seq technology

【背景】饱水保藏木漆器的环境中,微生物数量多、种类丰富,木漆器易受到微生物的腐蚀。【目的】研究木漆器上的微生物群落结构,分析饱水木漆器的微生物病害信息。【方法】采用Illumina MiSeq高通量测序技术对木漆器和保藏水样中的细菌进行群落结构分析。【结果】木质样品与水样品中的微生物群落多样性较丰富并在分布上存在一定差异。门水平上,木质样品共有7个优势菌门(相对丰度>1%),分别为Proteobacteria(64.00%)、Acidobacteria(14.70%)和Actinobacteria(3.83%)等;水样共有6个优势菌门,分别为Proteobacteria(61.26%)、Acidobacteria(8.25%)和Planctomycetes(4.88%)等。属水平上木质样品共有8个优势菌属(相对丰度>1%),分别为Phenylobacterium(16.24%)、Acidobacteria-Gp6(9.68%)和Rhodoplanes(6.45%)等;水样共有10个优势菌属,分别为Naxibacter(9.03%)、Acidobacteria-Gp6(3.84%)和Nevskia(3.27%)等。未分类菌在门和属上,木质样品分别占8.70%和50.68%;水样品分别占12.83%和59.35%。【结论】木漆器饱水保藏环境中木质样品与水样品的微生物群落结构均比较丰富,在门和属水平上水样品比木质样品复杂。另外,被测样品中可能含有大量潜在新菌。

[Background] The wood lacquer is vulnerable to microbial corrosion in the environment of water preserved wood lacquer because of microorganisms. [Objective] In order to study the microbial community structure and microbial disease information of wood lacquer ware. [Methods] The community structure of bacteria in wood lacquer and water samples was analyzed by Illumina MiSeq high throughput sequencing technology. [Results] There were differences between wood samples and water samples in the microbial community structure on the gate level, wood samples of a total of 7 dominant bacteria（relative abundance, gate1%） were Proteobacteria（64.00%）, Acidobacteria（14.70%） and Actinobacteria（3.83%）. There were 6 dominant phyla detected at the phylum level, Proteobacteria（61.26%）, Acidobacteria（8.25%） and Planctomycetes（4.88%）, respectively. A total of 8 dominant species（relative abundance1%） were found in the woody samples, which were Phenylobacterium（16.24%）, Acidobacteria-Gp6（9.68%） and Rhodoplanes（6.45%）, respectively. There were 10 dominant genera in water samples, which were Naxibacter（9.03%）, Acidobacteria-Gp6（3.84%） and Nevskia（3.27%）, respectively. The unclassified bacteria in wood samples accounted for 8.70% and 50.68%, respectively. The unclassified bacteria in water samples accounted for 12.83% and 59.35% respectively in the door and genus. [Conclusion] The microbial community structure of wood samples and water samples is abundant in the water preservation environment of wood lacquerware, but the microbial diversity is more complex than that of wood samples in the water samples of the door and genus level. In addition, a large number of potential new bacteria were detected in the tested samples.