丝状真菌蛋白质组学研究进展

Research progress on proteomics in filamentous fungi

丝状真菌不仅是致病菌,而且在异源表达工业酶、化学制品以及药物活性物质中发挥着越来越重要的作用。随着人类基因组计划的实施和推进,生命科学研究已进入了功能基因组时代,特别是蛋白质组学,在蛋白质水平对丝状真菌细胞生命过程中蛋白质功能和蛋白质之间的相互作用以及特殊条件下的变化机制进行研究,对生命的复杂活动进行深入而又全面的认识也为丝状真菌工业酶制剂和重组药物的开发提供广阔的创新空间。本文综述了蛋白质组学的研究内容和方法,总结了其在丝状真菌致病菌、抗生素产生菌和纤维素酶产生菌中的应用现状。不同层次的功能基因组学分析可以从各个角度掌握生物体的代谢网络和调控机制,本文还对蛋白质组学以及功能基因组学各部分内容的整合运用进行了展望。

Filamentous fungi not only are pathogenic but also play a significant role in heterologous expressing enzymes, chemicals, and pharmaceuticals. Along with the implementation and promotion of Human Genome Project, life science has entered functional genomics era. Proteomics study is a type of functional genomics research in order to understand cellular process mechanism, protein functions and their interactions, which provides a vast potential for future production of industrial enzymes and recombinant pharmaceutical proteins by filamentous fungi. This review summarized the research contents and methods of proteomics study, and its applications in filamentous fungi including pathogenic, antibiotic producing and cellulase producing strains. Furthermore, future studies in transcriptomics, proteomics and metabolomics could offer a full scale of understanding the metabolic network and regulations in filamentous fungi.