绿色荧光蛋白标记的Lewis肺癌小鼠原位模型的建立及鉴定

Establishment and Confirmation of GFP-Labled Orthotopic Lewis Lung Cancer Mouse Model

目的：建立具有免疫功能的小鼠原位荷瘤模型,并优化其评价方式。方法：通过注射绿色荧光蛋白（GFP）标记的Lewis肺癌细胞（LLC）悬液至C57BL/6小鼠肺部。方法：利用表达GFP基因的病毒转染Lewis肺癌细胞。采用肿瘤细胞悬液注射法建立Lewis肺癌原位模型。小动物活体成像系统鉴定其成瘤情况。CT薄层扫描和HE染色确定其位置和病理性质。结果：在荷瘤2周之后CT显示肺部有占位,小鼠胸部可观察到绿色荧光信号,HE染色确定其为肿瘤组织。结论：GFP标记的Lewis肺癌小鼠原位模型的建立和评估是可行的,为肺癌的基础研究提供了有效的动物模型。

Objective：To establish an orthotopic mouse model with normal immune function,and to optimize the evaluation system for this model.Methods：Lewis lung carcinoma cells were transfected with the GFP lentiviruses,then,these GFP-labeled cancer cell suspension was orthotopically injected into the lungs of C57 BL/6 mice.The growth of the orthotopic tumor was analyzed by an in vivo fluorescence imaging system for small animal.Thin-Layer Scanning CT was used to identify the tumor loci,and pathological characteristics were examined by hematoxylin and eosin（HE）staining.Results：Occupying lesion was observed on the images of CT scan 2 weeks after orthotopic injection.Green fluorescence was visible through the flap,and cancer was confirmed by HE staining.Conclusion：The establishment and evaluation of GFP-labeled orthotopic Lewis lung cancer mouse model is feasible.It provides an effective platform for basic research of lung cancer.