侧向层析技术标记物研究进展

Research advance of lateral flow assay labels

侧向层析技术以其简单、快速、灵敏、经济等优势广泛应用于即时检验、现场检验以及家庭自检中。合适的标记物的选择对整个实验的灵敏度起着重要作用。传统的标记物有胶体金、纳米硒以及纳米碳等,尤以胶体金最为常用。近年来新型标记物的出现,如量子点、纳米酶等,使得侧向层析有了进一步的发展,实现了从定性检测逐步向定量检测的转变。本文旨在介绍目前已有的侧向层析标记物,为侧向层析工作者筛选合适的标记物提供一定的研究依据。

Lateral flow assay is widely used in the point-of-care testing on-site and in-home testing with the advantage of being simple, rapid, sensitive and cost-effective. Proper labels are the key factors in lateral flow assay.Traditional labels include colloidal gold, selenium nanoparticle, and carbon nanoparticle, among which the colloidal gold is most commonly used. Lateral flow assay has been improved as a result of the discovery of new labels, such as quantum dots and nanozyme recently. Meanwhile, transformation of qualitative detection to quantitative detection is gradually realized. This article aims at introducing the most often used and the latest lateral flow assay labels, providing a basis theoretical investigation on screening proper labels for lateral flow assay researchers.