FokI rs2228570和TMPRSS6 rs855791多态性与儿童牛奶蛋白过敏的相关性

Association of FokI rs2228570 and TMPRSS6 rs855791 polymorphisms with cow's milk protein allergy in children

目的探讨维生素D受体(VDR)基因FokI rs2228570及TMPRSS6 rs855791基因多态性与儿童牛奶蛋白过敏(CMPA)的关系。方法应用实时荧光定量PCR检测100例CMPA儿童及100例健康儿童(对照组)VDR基因FokI rs2228570和TMPRSS6 rs855791单核苷酸多态性。使用多因素logistic回归模型评估CMPA发生的危险因素。结果 CMPA组与对照组TMPRSS6 rs855791基因型CC、CT、TT分布频率差异有统计学意义(P=0.008),CMPA组的TT基因型占优势。多因素logistic回归分析提示rs855791 TT基因型的儿童发生CMPA的风险增加(OR=3.473,P=0.011)。而VDR基因FokI rs2228570基因型分布在两组儿童间的差异无统计学意义(P=0.686)。结论 TMPRSS6 rs855791多态性与儿童CMPA的发生有关联,TT基因型可能为儿童CMPA的易感基因型,而VDR基因FokI rs2228570多态性与儿童CMPA无关联。

Objective To study the association of polymorphisms of FokI rs2228570 in the vitamin D receptor（VDR） gene and TMPRSS6 rs855791 with cow＇s milk protein allergy（CMPA） in children. Methods Quantitative realtime PCR was used to analyze the single nucleotide polymorphisms of FokI rs2228570 in the VDR gene and TMPRSS6 rs855791 in 100 children with CMPA and 100 healthy children（control group）. The multivariate logistic regression model was used to identify the risk factors for CMPA. Results There were significant differences in the frequencies of CC, CT, and TT genotypes of TMPRSS6 rs855791 between the CMPA and control groups（P=0.008）, and the CMPA group had a significantly higher frequency of TT genotype. The multivariate logistic regression analysis showed that the children with TT genotype of rs855791 had an increased risk of CMPA（OR=3.473, P=0.011）. However, there was no significant difference in the genotype distribution of FokI rs2228570 in the VDR gene between the two groups（P=0.686）. Conclusions TMPRSS6 rs855791 polymorphism is associated with CMPA in children, and TT genotype may be the susceptible genotype of CMPA. FokI rs2228570 polymorphism is not associated with CMPA.