摘要
目的:研究深圳龙华区0~5岁门诊婴幼儿腹泻轮状病毒(RV)分型A组感染现状及基因型流行特征,为防治RV性腹泻提供科学依据。方法:收集1033例腹泻婴幼儿的1033份粪便标本,按年龄将其分为<0.5岁组(125例)、0.5~1岁组(492例)、1~3岁组(305例)和>3岁组(111例)4组。对1033份粪便标本采用胶体金免疫层析法检测RV抗原,采用巢式-聚合酶链反应(PCR)法及测序对A组RV阳性标本进行血清型分型,并统计分析A组RV感染季节分布及A组RV的G血清型和P血清型分布情况。结果:在1033份婴幼儿腹泻标本中检出A组RV抗原436份,阳性率为42.21%,男性略高于女性,但无明显差异。0.5~1岁组婴幼儿A组RV感染率最高(占61.18%),与其他3组比较差异有统计学意义(x^2=9.762,P<0.05)。感染季节以第4季度A组RV感染率为最高(占53.54%),与第1季度比较无明显差异,但与第2和第3季度比较差异有统计学意义(x^2=4.135,x^2=5.415;P<0.05)。RV的G血清型以G3血清型检出率最高(占53.21%),与G1、G2、G4和G9血清型比较差异有统计学意义(x^2=3.854,x^2=6.751,x^2=11.582,x^2=19.192;P<0.05);混合感染以G3G1血清型为主,占55.0%;RV的P血清型中以P[8]血清型检出率最高(占79.82%),与P[1]、P[4]、P[9]和P[6]血清型比较差异有统计学意义(x^2=27.025,x^2=31.635,x^2=40.924,x^2=49.516;P<0.05);混合感染以P血清型为主,占52.94%;RV的G和P血清型混合感染主要为G3P[8]和G1P[8]血清型,其阳性率分别为43.58%和28.90%。结论:A组RV是导致深圳龙华区0~5岁门诊婴幼儿腹泻主要病原体之一,好发于秋、冬季节和<1岁婴幼儿。基因型流行呈多样性变化,G3血清型、P[8]血清型、G3P[8]和G1P[8]血清型为本区主要流行基因型。
Objective: To understand the current situation and genotype epidemiological feature of rotavirus(RV) A group infection of diarrhea infants whose age were between 0-5 years old in Longhua District of Shenzhen city so as to provide scientific basis for preventing diarrhea caused by RV. Methods: 1033 fecal samples of 1033 infants with diarrhea were collected and were divided into 4 groups included less than 0.5 years old group(125 cases), 0.5-1 years old group(492 cases), 1-3 years old group(305 cases) and higher than 3 years group(111 cases). The collaurum immunochromatographic method was adopted to detect RV antigen for 1033 fecal samples, and the nested-PCR and sequencing were adopted to implement serotype for samples with positive A group RV. And then the seasonal distribution of A group RV infection and the distribution of G serotype and P serotype of A group RV were further analyzed by using statistical method. Results: In 1033 samples, 436 samples were found RV antigen and the positive rate was 42.21%, the rate of male was slightly higher than that of female, while the difference was no significant. The infection rate of A group RV of 0.5-1 years old group was highest(61.18%), and the difference of infection rate between it and any other groups was significant(x2=9.762, P〈0.05). And the infection rate of the fourth quarter was highest(53.54%), and the differences of infection rate between the fourth quarter and the second quarter and between the fourth quarter and the third quarter were significant(x2=4.135, x2=5.415, P〈0.05), respectively, although the difference of infection rate between the fourth quarter and the first quarter was no significant. The relevance ratio of G3 serotype in the G serotype of RV was highest(53.21%), and the differences of relevance ratio between G3 and G1, G2, G4, G9, respectively, was significant(x2=3.854, x2=6.751, x2=11.582, x2=19.192, P〈0.05). The G3 G1 serotype(55.0%) was main in mixed infection, and the relevance rate of P[8] serotype(79.82%) in P serotype was highest, and the differences of relevance rate between P[8] and P[1], P[4], P[9], P[6], respectively was significant(x2=27.025, x2=31.635, x2=40.924, x2=49.516, P〈0.05). In mixed infection, the P serotype was main(52.94%). Besides, the G3 P[8] and G1 P[8] were the mainly mixed infections of G serotype and P serotype, and their positive rates were 43.58% and 28.90%, respectively. Conclusion: A group RV is one of main pathogens causing diarrhea in infants(0-5 years old) in Longhua district of Shenzhen. Autumn and winter commonly are onset seasons, and infants whose age is less than 1 year old are susceptible population. Genotypic prevalence shows a diversified transformation, and G3, P[8], G3 P[8] and G1 P[8] serotypes are the mainly popular genotypes in this district.
作者
刘爱玲
刘爱胜
吴敏
LIU Ai-ling;LIU Ai-sheng;WU Min(Clinical Laboratory,The People's Hospital of Longhua District of Shenzhen,Shenzhen 518109,China.)
出处
《中国医学装备》
2018年第8期85-89,共5页
China Medical Equipment
基金
深圳市龙华区科技局基金(708057661031)
深圳市龙华新区2015年科技创新基金"重点实验室"项目资助(2015-39)"轮状病毒(RV)所致婴幼儿腹泻的检测及其基因分型"
关键词
婴幼儿
腹泻
A组轮状病毒
感染
基因分析
Infant
Diarrhea
A group rotavirus
Infection
Genetic analysis