桂枝茯苓丸对子宫内膜异位症大鼠血清免疫球蛋白和炎症因子水平的影响

The effect of Guizhi Fuling pills on serum immunoglobulin and inflammatory factor in rats with endometriosis

目的探讨桂枝茯苓丸对子宫内膜异位症大鼠血清免疫球蛋白和炎症因子水平的影响。方法采用自体移植法建立子宫内膜异位症大鼠模型,将造模成功的60只大鼠随机分为模型对照组、阳性对照组,以及桂枝茯苓丸低、中、高剂量组,并选10只大鼠设为空白对照组,阳性对照组给予38mg/kg的达那唑混悬液灌胃,桂枝茯苓丸组分别给予0.62、1.24、2.17g/kg的剂量灌胃,模型对照组和空白对照组给予相同体积的消毒饮用水灌胃,灌胃4周后,采用散射比浊法和ELISA双抗体夹心法分别检测大鼠血清免疫球蛋白IgG、IgA、IgM和白细胞介素(IL)-6、IL-8水平。结果模型对照组血清IgG、IgA、IgM、IL-6和IL-8含量明显高于空白对照组(P<0.05);与模型对照组比较,中、高剂量桂枝茯苓丸组IgG、IgA、IgM含量显著降低(P<0.05);高剂量桂枝茯苓丸组可显著降低大鼠血清中IL-6和IL-8水平(P<0.05)。结论桂枝茯苓丸可以调节子宫内膜异位症大鼠的免疫功能,对子宫内膜异位症大鼠具有明显的治疗作用。

Objective To explore the effect of guizhi fuling pills in serum immunoglobulin and inflammatory factor in rats with endometriosis.Methods A rat endometriosis model was established by autologous grafting operation method.60 rats with successfully established model were randomly divided into the model control group,the positive control group,guizhi fuling pills groups with low-,middle-and high-dose,and another10 rats were set as the blank control group.Rats in positive control group were given 38 mg/kg of danazol suspensions by gavage.Rats in guizhi fuling pills group were given its suspensions with the dose of 0.62 g/kg,1.24 g/kg and 2.17 g/kg by gavage.Rats in the model control group and the blank control group were only given sterilize water by gavage.After 4 weeks,the levels of IgG,IgA,IgM and IL-6,IL-8 in serum were measured by immunoturbidimetric method and ELISA double antibody sandwich method,respectively.ResultsCompared with the blank control group,the serum levels of IgG,IgA,IgM,IL-6 and IL-8 in the model control group were significantly higher（P〈0.05）.Compared with the model control group,the serum levels of IgG,IgA and IgM in guizhi fuling pills group with middle-and high-dose group were significantly lower（P〈0.05）.The levels of IL-6 and IL-8 in serum were down regulated by high-dose guizhi fuling pills（P〈0.05）.Conclusion Guizhi fuling pills could regulate the body′s immune function in the experimental rats with endometriosis,and this treatment has an obvious curative effect on rats with endometriosis.