人乳头瘤病毒感染及p53、P16、Cdc2表达与喉癌预后的关系

Relationship between human papillomavirus infection,p53,P16,Cdc2 expressions and laryngeal cancer prognosis

目的研究人乳头瘤病毒(HPV)感染及p53、P16、Cdc2表达与喉癌预后的关系。方法选择2012年6月至2013年5月在该院保留完整随访资料的喉癌手术切除标本62例,分析HPV感染和p53、P16、Cdc2表达在喉癌组织中的阳性表达情况和预后关系,肿瘤分化程度及分期和喉癌患者复发的关系。结果 HPV感染在喉癌组织中以高危型HPV-16为主,通过2年随访,发现喉癌患者中有50例未复发,12例出现复发。喉癌组织中HPV感染率为19.35%(12/62),在喉癌组织中p53阳性表达率为59.68%(37/62),P16阳性表达率为58.06%(36/62),Cdc2阳性表达率为64.52%(40/62)。HPV感染和p53、P16、Cdc2表达和喉癌的分化程度及临床分期无明显相关性(P>0.05),HPV阳性喉癌患者术后复发率[0.00%(0/12)]显著低于无复发患者[24.00%(12/50)],差异有统计学意义(P<0.05)。复发组喉癌患者的p53阳性表达率[58.33%(7/12)]和未复发组[60.00%(30/50)],差异无统计学意义(P>0.05)。复发组喉癌患者的P16阳性表达率[83.33%(10/12)]显著高于未复发组[52.00%(26/50)],差异有统计学意义(P<0.05)。复发组喉癌患者的Cdc2阳性表达率[100.00%(12/12)]显著高于未复发组[56.00%(28/50)],差异也有统计学意义(P<0.05)。经Spearman等级相关分析得知,肿瘤分化程度及分期和喉癌患者的复发无明显相关性(P>0.05)。结论HPV感染、p53、P16、Cdc2表达和喉癌的预后存在一定关联性。

Objective To study the relationship between human papillomavirus infection（HPV）,p53,P16,Cdc2 expressions and prognosis of laryngeal carcinoma.Methods 62 cases of laryngeal carcinoma resection were retrospectively analyzed from June 2012 to May 2013.The positive expression and prognosis of HPV infection and p53,P16 and Cdc2 expressions in laryngeal squamous cell carcinoma,and the degree of tumor differentiation and staging and the recurrence of and laryngeal cancer were analyzed.Results HPV-16 was the main type of HPV infection in laryngeal squamous cell carcinoma.By two-year follow up,50 patients with laryngeal cancer were found to have no recurrence and recurrence occurred in 12 cases.The positive rate of HPV infection in laryngeal squamous cell carcinoma was 19.35%（12/62）.The positive rate of p53 expression in laryngeal squamous cell carcinoma was 59.68%（37/62）,the positive expression rate of P16 was 58.06%（36/62）,and the positive rate of Cdc2 was 64.52%（40/62）.There was no significant correlation between HPV infection and p53,P16 and Cdc2 expressions and differentiation and clinical stage of laryngeal carcinoma（P〉0.05）.The recurrence rate of HPV-positive laryngeal cancer patients was significantly lower than that of patients without recurrence[0.00%（0/12）vs.24.00%（12/50）],and the differences were statistically significant（P〈0.05）.There was no significant correlation between the positive rate of p53 expression in patients with recurrent laryngeal cancer and without recurrence[58.33%（7/12）vs.60.00%（30/50）]（P〉0.05）.The positive rate of P16 in patients with recurrent laryngeal carcinoma was significantly higher than that in patients without recurrence[83.33%（10/12）vs.52.00%（26/50）]and the differences were statistically significant（P〈0.05）.The positive expression rate of Cdc2 was significantly higher than that in patients without recurrence[100.00%（12/12）vs.56.00%（28/50）],and the difference was statistically significant（P〈0.05）.Spearman rank correlation analysis showed that there was no significant correlation between tumor differentiation and the stage and recurrence of laryngeal carcinoma（P〉0.05）.Conclusion The expressions of p53,P16 and Cdc2 in HPV infection and the prognosis of laryngeal carcinoma are related.