趋化因子受体7在食管鳞状细胞癌组织中表达的相关研究

The correlation study of expression of chemokine receptor 7 in esophageal squamous cell carcinoma

目的探讨趋化因子受体7(CXCR7)在食管鳞状细胞癌(食管鳞癌)组织中的表达及其临床意义。方法收集2014年1月至2015年12月新疆维吾尔自治区人民医院手术切除的食管癌组织蜡块标本60例,经病理证实癌组织类型为鳞癌。采用免疫组化S-P法,按照试剂盒操作说明书检测食管鳞癌及癌旁正常组织中CXCR7的表达,分析食管鳞癌组织中CXCR7的表达与患者临床病理特征的关系。结果在食管鳞癌组织中,CXCR7主要定位于细胞质和细胞膜中,呈淡黄色或棕黄色颗粒。食管鳞癌组织中CXCR7的阳性表达率为71.2%(43/60),阴性表达率为28.3%(17/60),癌旁组织中CXCR7的阳性表达率仅为6.7%(4/60),CXCR7在癌旁组织中阴性表达率为93.3%(56/60)。CXCR7在食管癌组织中的阳性表达率高于癌旁组织,差异有统计学意义(χ2=53.197,P=0.000)。CXCR7表达与肿瘤TNM分期及淋巴结转移有关(P<0.05)。发生淋巴结转移、TNM分期为Ⅱ~Ⅲ期的食管癌患者CXCR7的阳性表达率高于未发生淋巴结转移、TNM分期为Ⅰ期的食管癌患者,差异有统计学意义(P<0.05)。采用COX回归分析显示,肿瘤淋巴结转移和CXCR7的表达为食管癌独立的预后影响因素。Kaplan-Meier分析显示,患者CXCR7的表达高,生存时间更短,低CXCR7表达的患者生存时间更长(P=0.000)。结论 CXCR7可能参与了食管癌发展和淋巴结转移过程。

Objective To investigate the expression of chemokine receptor 7（CXCR7）in esophageal squamous cell carcinoma and its clinical significance.Methods Sixty patients with esophageal squamous cell carcinoma in Xinjiang Uygur Autonomous Region from January 2014 to December 2015 were enrolled.The type of squamous cell carcinoma was confirmed by pathology.Immunohistochemical S-P method was used to detect the expression of CXCR7 in esophageal squamous cell carcinoma and adjacent normal tissues.The relationship between the expression of CXCR7 in esophageal squamous cell carcinoma and clinicopathological features were analyzed.Results In esophageal squamous cell carcinoma,CXCR7 was mainly located in the cytoplasm and cell membrane,with pale yellow or brown particles.The positive expression rate of CXCR7 in esophageal squamous cell carcinoma was 71.2%（43/60）and negative expression rate was 28.3%（17/60）.The positive expression rate of CXCR7 in paracancerous tissue was only 6.7%（4/60）The expression of CXCR7 in the adjacent tissues was 93.3%（56/60）.The positive expression rate of CXCR7 in esophageal carcinoma tissues was significantly higher than that in adjacent tissues,and the difference was statistically significant（χ^2=53.197,P=0.000）.The expression of CXCR7 was correlated with TNM stage and lymph node metastasis（P〈0.05）.The positive rate of CXCR7 expression in patients with lymph node metastasis,stageⅡ-Ⅲ TNM was significantly higher than that in patients with no node metastasis and stage Ⅰ,and the difference was statistically significant（P〈0.05）.COX regression analysis showed that lymph node metastasis and CXCR7 expression were independent prognostic factors for esophageal cancer.Kaplan-Meier analysis showed that patients with high expression of CXCR7 and shorter survival time,and low CXCR7 expression in patients with longer survival time（P=0.001）.Conclusion CXCR7 may be involved in the development of esophageal cancer and lymph node metastasis.