疫区人群树突状细胞CD209启动子多态性及其与登革病毒感染的相关性分析

Analysis of polymorphism of CD209 promoter in dendritic cells and its association with dengue virus infection in epidemic areas

目的以深圳疫区人群为研究对象,分析CD209启动子(-139、-336)多态性与登革病毒(DENV)感染之间的相关性。方法收集疫区DENV感染者及健康对照者的抗凝血标本,提取血液基因组DNA,PCR扩增CD209启动子区基因判断,采用Spe I酶切、错配PCR及DNA测序等方法对-139及-336位点的多态性进行分析,采用Fisher′s检验分别计算CD209-139位点及-336位点不同基因型及等位基因型与DENV感染的相关性。结果以深圳市人群为对象,登革病毒感染者在CD209启动子-139位点出现A等位基因的频率为74.5%(76/102),显著高于健康人群(43.8%,89/203),差异有统计学意义(P<0.05),提示疫区人群CD209-139A与DENV感染明显相关(OR=1.659,95%CI:1.017~2.706,P<0.05);-336基因多态性则与DENV易感性没有显著相关性。结论疫区人群CD209启动子-139A可能会增加DENV易感性。

Objective To elucidate the correlations between promoter polymorphisms（-139 and-336 sites）of human dendritic cell CD209 and Dengue virus infection among the people in Shenzhen epidemic area.Methods Blood samples of both DENV infected patients and healthy controls were collected,from which the genome DNA was extracted.Partial sequences of CD209 promoter region were amplified by PCR,and the polymorphisms of-139 and-336 sites were analyzed by Spe I digestion,error-prone PCR or DNA sequencing.Finally,analysis of correlations between CD209 promoter polymorphisms and DENV infection was performed by Fisher′s exact test.Results Among the people in Shenzhen epidemic area,the-139 Aallele had a frequency of 74.5%（76/102）in individuals with DENV infection,which was significantly higher than that in healthy controls（43.8%,89/203）,and the difference was statistically significant（P〈0.05）,suggesting a strong association between CD209/-139 Aand the risk of DENV infection（OR=1.659,95%CI：1.017-2.706）.-336 genetic polymorphisms were not significantly correlated with DENV susceptibility.Conclusion CD209/-139 A was strongly associated with DENV susceptibility among the people in epidemic area.