印度黄檀干热河谷扦插试验研究

A Study of the Cutting Rooting of Dalbergia sissoo in Dry-hot Valleys

在四川金沙江干热河谷区,通过采用不同植物生长调节剂(IBA、NAA、IAA、6-BA)对印度黄檀的扦插繁殖进行了对比试验研究。试验结果显示,无论何种处理印度黄檀都有较明显的愈伤组织产生。就生根率而言,IBA处理效果较为明显,IBA100 mg·L^(-1)与IBA200 mg·L^(-1)分别插条生根率达到了56%和66%的生根率,但是IBA400mg·L^(-1)与IBA800 mg·L^(-1)处理生根率却只有30%和18%,浓度过高反而不利于愈伤组织形成和根的生长;NAA处理中,只有NAA100 mg·L^(-1)较为明显,生根率达到44%,而IAA处理中,生根率普遍不高,生根率为20%~34%。对照水浸泡处理生根率只有12%,而6BA处理均没有发现生根。试验中的6BA处理和NAA25 mg·L^(-1)处理没有发现生根,也可能是由于试验的其他不可控因素导致,有待进一步研究。

Comparative studies were made of cutting experiments of Dalbergia sissoo treated by different plant-growth regulations including IBA,NAA,IAA,6-BA in dry-hot valleys of Panzhihua.The results showed that visible callus occurred under all the above treatments.IBA could significantly improve the rooting rate of D.sissoo .The rooting ratios of cuttings were up to 56% and 66% respectively by treatment of IBA 100 mg·L^-2 and IBA 200 mg·L^-2 ,better than other IBA treatments,the rooting ratios of cuttings were 30% and 18% with IBA 400 mg·L^-2 and IBA 800 mg·L^-2 treatments.Obviously,the high concentration of IBA could inhibit the growth of root tissue cultures.The highest rooting ratio reached 44% by NAA 100 mg·L^-2 treatment,but rooting ratios were 20% to 34% by all the treatments of IAA.There was no rooting occurred under all the 6BA treatments and 25 mg·L^-2 NAA,even lower than that of control group,which could be influenced by other uncontrollable factors for further research.