骨髓液中FGF-1、Smad3表达与再生障碍性贫血发病的关系

Relationships between Expression of FGF-1 and Smad3 in Bone Marrow Fluid and Pathogenesis of Aplastic Anemia

目的探讨骨髓液中成纤维细胞生长因子-1(FGF-1)、Smad3表达与再生障碍性贫血(AA)发病的关系。方法选取2013年2月-2016年1月我院收治的50例AA患者作为研究组,选择同期来我院体检的40例健康者作为对照组,均行髂后上嵴穿刺,抽取骨髓液5 ml,测定FGF-1、转化生长因子β1(TGF-β1)、Smad3表达情况,分析FGF-1、Smads表达与AA发病的关系。结果研究组患者MSC中TGF-β1及Smad3蛋白表达水平均低于对照组(P<0.05);研究组骨髓MSC分泌细胞培养上清中TGF-β1、FGF-1 ELISA检查结果均低于对照组(P<0.05);AA患者骨髓MSC培养4 d后即出现大量脂滴细胞,滴加TGF-β1、FGF-1培养20 d后均未出现或少见脂滴细胞。结论 AA患者骨髓液FGF-1、TGF-β1、Smad3表达水平均较低,可诱导MSC脂质分化,添加FGF-1、TGF-β1可抑制AA患者MSC向脂肪细胞分化。

Objective To investigate the relationships between the expressions of fibroblast growth factor-1（ FGF-1）and Smad3 in bone marrow fluid and the pathogenesis of aplastic anemia（ AA）.Methods A total of 50 patients with AA treated in our hospital between February 2013 and January 2016 were selected as the study group,while another 40 healthy people were chosen as the control group. All the subjects underwent posterior superior spine puncture. 5 ml of bone marrow fluid was extracted to determine the expressions of FGF-1,transforming growth factor-β1（ TGF-β1） and Smad3. The relationships between the expressions of FGF-1 and Smads and the pathogenesis of AA were analyzed. Results The expression levels of TGF-β1 and Smad3 protein in MSC of the study group were lower than those in the control group（ P〈0.05）. The ELISA results of TGF-β1 and FGF-1 in the study group were lower than those in the control group（ P〈0.05）. A large number of lipid droplets were found in the bone marrow of patients with AA after 4 days of MSC culture,but few or no lipid droplets were found after 20 days of culture with TGF-β1 and FGF-1 added.Conclusion The expression levels of FGF-1,TGF-β1 and Smad3 in bone marrow fluid of patients with AA are low,which can induce the differentiation of MSC lipids. The addition of FGF-1 and TGF-β1 can inhibit the differentiation of MSC from patients with AA into adipocytes.