白细胞介素23受体基因多态性与广西壮族人群骨关节结核的易感性

Polymorphisms of interleukin-23 receptor gene and susceptibility of bone and joint tuberculosis in Guangxi Zhuang populations

背景:广西壮族自治区是结核病的高发地域,大量研究证实结核病存在易感基因。目的:分析白细胞介素23受体基因多态性及其与广西壮族人群骨关节结核易感性之间的关系。方法:选取2014年7月到2017年3月右江民族医学院附属医院住院的125例骨关节结核患者及150例健康体检者作为研究对象,利用PCR扩增技术和DNA测序方法分别对骨关节结核患者及健康体检者白细胞介素23受体rs10489628G/A、rs10489629T/C、rs10889675A/C、rs10889677C/T、rs7530511C/A位点进行多态性检测,比较2组基因型以及等位基因的频率分布差异,探讨各基因型与骨关节结核易感性的关系。结果与结论:白细胞介素23受体基因rs10489629 T/C位点骨关节结核组C等位基因频率29.2%,高于对照组(21.3%);rs10889675 C/A位点骨关节结核组A等位基因频率26.0%,高于对照组(18.0%),骨关节结核组CA频率37.6%高于对照组(25.3%);差异均有显著性意义(P<0.05)。骨关节结核组单倍型GTCAC频率69.6%低于对照组(77.3%)、ACACC频率26.0%高于对照组(17.7%)。提示白细胞介素23受体rs10489629T/C、rs10889675C/A位点多态性可能与广西壮族人群骨关节结核易感性有关。

BACKGROUND： The incidence of tuberculosis is high in populations from Guangxi Zhuang Autonomous Region. Tuberculosis has been shown to have susceptibility gene.OBJECTIVE： To analyze the relationship between interleukin-23 receptor（IL-23 R） gene polymorphism and susceptibility to bone and joint tuberculosis in Guangxi Zhuang populations. METHODS： Totally 125 patients with bone and joint tuberculosis admitted in the Affiliated Hospital of Youjiang Medical University for Nationalities and 150 healthy subjects were selected. The gene polymorphism of IL-23 R（rs10489628 G/A, rs10489629 T/C, rs10889675 A/C, rs10889677 C/T, rs7530511 C/A） was analyzed by PCR amplification technique and DNA sequencing method. The genotypes and frequency distribution of alleles were compared between the tuberculosis and control groups. The relationship between IL-23 R gene polymorphism and the susceptibility to bone and joint tuberculersis was discussed. RESULTS AND CONCLUSION： The frequency of C allele of IL-23 R rs10489629 T/C in the tuberculosis group was 29.2%, which was significantly higher than that in the control group（21.3%）（P〈0.05）. The frequency of A allele of IL-23 R rs10889675 C/A in the tuberculosis group was 26.0%, which was significantly higher than that in the control group（18.0%）. The frequency of CA genotype of IL-23 R rs10889675 C/A in the tuberculosis group was 37.6%, which was significantly higher than that in the control group（25.3%）（P〈0.05）. The frequency of GTCAC haplotype in the bone and joint tuberculosis group was 69.6% which was lower than that in the control group（77.3%）. The frequency of ACACC haplotype in the bone and joint tuberculosis group was 26.0% which was higher than that in control group（17.7%）. In summary, the gene polymorphism of IL-23 R rs10489629 T/C and rs10889675 C/A may be related to the susceptibility of bone and joint tuberculosis in Guangxi Zhuang populations.