色素上皮源性因子局部注射对糖尿病大鼠视网膜内血管新生、炎症反应及细胞凋亡的影响

Effect of local injection of pigment epithelium-derived factor on angiogenesis,inflammatory response and apoptosis in the retina of diabetic rats

目的:研究色素上皮源性因子(PEDF)局部注射对糖尿病大鼠视网膜内血管新生、炎症反应及细胞凋亡的影响。方法:选择成年雄性SD大鼠作为实验动物,随机分为对照组、DM组、PEDF组,DM组、PEDF组通过链脲佐菌素腹腔注射的方式建立糖尿病模型,PEDF组给予PEDF局部注射。干预后测定视网膜组织中血管新生分子、炎症反应分子的含量以及细胞凋亡基因的表达量。结果:DM组大鼠视网膜组织中mTOR、VEGF、VEGFR、Ang-2、Tie-2、NF-κB、COX2、iNOS、SDF-1、CXCR4的含量以及ASK1、JNK、p38MAPK、Caspase-3的mRNA表达量均显著高于对照组,Survivin、Bcl-2的mRNA表达量均显著低于对照组;PEDF组大鼠视网膜组织中mTOR、VEGF、VEGFR、Ang-2、Tie-2、NF-κB、COX2、iNOS、SDF-1、CXCR4的含量以及ASK1、JNK、p38MAPK、Caspase-3的mRNA表达量均显著低于DM组,Survivin、Bcl-2的mRNA表达量均显著高于对照组。结论:PEDF局部注射对糖尿病大鼠视网膜内血管新生、炎症反应及细胞凋亡均具有抑制作用。

Objective：To study the effect of local injection of pigment epithelium-derived factor（PEDF）on angiogenesis,inflammatory response and apoptosis in the retina of diabetic rats.Methods：Adult male SD rats were selected as experimental animals and randomly divided into control group,DM group and PEDF group,DM group and PEDF group were made into diabetes models through intraperitoneal injection of streptozotocin,and PEDF group were given local injection of PEDF.The contents of angiogenesis molecules and inflammatory response molecules as well as the expression of apoptosis genes in retinal tissue were measured after intervention.Results：mTOR,VEGF,VEGFR,Ang-2,Tie-2,NF-κB,COX2,iNOS,SDF-1 and CXCR4 contents as well as ASK1,JNK,p38 MAPK and Caspase-3 mRNA expression in retinal tissue of DM group were significantly higher than those of control group whereas Survivin and Bcl-2 mRNA expression were significantly lower than those of control group（P〈0.05）;mTOR,VEGF,VEGFR,Ang-2,Tie-2,NF-κB,COX2,iNOS,SDF-1 and CXCR4 contents as well as ASK1,JNK,p38 MAPK and Caspase-3 mRNA expression in retinal tissue of PEDF group were significantly lower than those of DM group whereas Survivin and Bcl-2 mRNA expression were significantly higher than those of control group（P〈0.05）.Conclusion：Local injection of PEDF has inhibitory effect on the angiogenesis,inflammatory response and apoptosis in the retina of diabetic rats.