Er:YAG激光对口腔黏液腺腺泡组织形态学的影响

Effect of Er: YAG Laser on Morphology of Acinar Tissue of Oral Mucous Gland

目的观察Er:YAG激光对口腔黏液腺腺泡组织细胞形态学的影响。方法收集行外科手术治疗的口腔黏液腺囊肿患者32例,留取每例患者囊肿旁体积均匀的腺泡组织3块,随机分为空白对照组、1.0W激光能量照射组、2.0W激光能量照射组。对照组不做处理,两激光组均采用德国Fotona牙科双波长激光中的Er:YAG激光进行处理。将以上3组组织在离体10min内进行固定,经包埋、切片、HE染色后光镜下观察细胞形态学改变。结果光镜下观察显示,对照组未见明显腺泡细胞的萎缩及坏死;两个激光组均见有腺泡细胞萎缩,且2.0W激光组腺泡细胞萎缩数目较空白对照组及1.0W激光组增多,甚至出现腺泡细胞坏死,坏死细胞体积变小,胞核可见固缩及碎裂,胞质深蓝染或蓝黑染。两个激光组腺泡萎缩数量及萎缩率均高于对照组,差异均有统计学意义(P<0.05)。1.0W激光组腺泡萎缩率达51.6%±4.0%,2.0W激光组腺泡萎缩率达88.4%±2.6%,两组间比较差异有统计学意义(F=1930.4,P=0.000)。结论激光照射腺泡后出现了腺泡萎缩的形态学改变,且升高激光能量照射腺泡后可使腺泡萎缩数量增加。

Objective To observe the effect of Er：YAG laser on morphology of acinar tissue of oral mucous gland. Methods In this study 11 patients with oral mucoid cyst were collected for surgical treatment, and each patient was left with three acinar tissue adjacent to the cyst. The three acinar tissue were randomly divided into 3 groups：blank control group,1.0W laser group and 2.0W laser group. The control blank group did not do any treatment while samples in two laser groups were irradiated using Er：YAG laser.Samples in the three groups were fixed in 10 minutes and the morphological changes were observed under light microscope after embedding and HE staining. Results HE staining revealed that the control group showed no obvious atrophy and necrosis of acinar cells. Both of the two laser groups displayed atropied acinus and the its number in 1.0W group increased and there is even necrosis cells.Smaller and rounder cell bodies,fragmentation and pycnosis of black nuclei were found in the necrosis cells. Statistics indicated that the number of atrophied acinar and atrophy rate of acinar in two laser groups were all higher than those in blank control group, and the difference was statistically significant. The atrophied acinus rate in 1.0W laser group is 51.6%±4.0% while 2.0W laser group is 88.4%±2.6%, the difference between two kinds of laser group have statistically significant（F=1930.4,P=0.000）. Conclusion The atrophied morphological changes of acinus appeared after laser irradiation, besides, the amount of atrophic acinus can be increased by increasing laser energy.