尿液中THP、OPN与泌尿系结石的相关性研究

Correlation study between THP,OPN and urinary calculi

目的探讨尿液中塔-霍粘蛋白(THP)及骨桥蛋白(OPN)水平与泌尿系结石的相关性。方法收集2017年1-6月于湖南师范大学第一附属医院确诊为泌尿系结石患者和健康无结石者的清晨空腹尿标本各65例,将其分为结石组和对照组。留取清晨空腹尿10mL,采用酶联免疫分析试验测定THP、OPN值,并对结果进行比较分析。结果结石组患者尿液中THP水平为(26.01±4.65)mg/L,OPN水平为(170.59±27.32)μg/mL,均显著低于对照组的(29.41±2.42)mg/L、(192.70±16.44)μg/mL,两组差异具有统计学意义(P<0.05);经Pearson相关性分析,THP与OPN在尿液中的表达呈正相关(r=0.254,P<0.05);Logistic回归分析得出,尿液中THP、OPN升高为泌尿系结石发生的危险因素(P<0.05)。结论泌尿系结石患者尿液中THP、OPN与健康人群存在明显差异,二者代谢水平异常与泌尿结石的形成存在相关性。

Objective To explore the correlation between urinary calculi and two protein makers Tamm Harsefall protein （THP） and osteopontin （OPN）. Methods Totally 65 cases of morning fasting urine speci mens were collected from January 2017 to June 2017 at the First Affiliated Hospital of Hunan Normal Univer sity were divided into stone group and 65 healthy people were selected as control group. Urinary calculi and healthy people were collected 10 mL of fasting urine each in the morning,testing THP and OPN value with the method of enzyme linked immunoassay （ELISA） ,then analyzed the results respectively. Results The concen tration of THP in the urine of urolithiasis patients was （26. 01±4. 65）mg/L and the concentration of OPN was （170.59±27.32）μg/mL,which were lower than control group （29.41± 2. 42）mg/L, （192. 70± 16. 44） μg/mL,the differences were statistically significant （P〈0.05）. Through Pearson correlation analysis, there was a positive correlation between THP and OPN expression in urine （r=0. 254,P〈0.05）. Logistic regres sion analysis showed that THP and OPN in the urine were risk factors for urinary calculi. Conclusion The concentration of THP and OPN in urine of urinary calculi patients are significantly different from those of healthy people,and defective expression of THP and OPN in urine have association with urinary calculi.