不同光质下中华盒形藻蛋白质合成的SDS-PAGE初步分析

Preliminary SDS-PAGE analysis of protein synthesis in Biddulphia sinensis grown under different light quality

初步分析光质对海洋硅藻中华盒形藻蛋白质合成的影响，发现在红光、蓝光和白光下蛋白质合成的量和种类有所不同。并对硅藻ＳＤＳ－ＰＡＧＥ样品制备法作了改进，即增加１次甲醇冲洗步骤，通过加热／冷冻循环破碎硅藻细胞。

The effect of light quality on protein synthesis of marine diatom Biddulphia sinensis was pre liminarily investigated. Protein amounts and species synthesized in red, blue and white light were somewhat different. And, the proparation of diatom protein sample for SDS-PAGE was improved, i. e., adding a methanol-washing step, breaking diatom cells by 2 cycles of heating/freezing.