胡杨基因组片段转化拟南芥表型研究

Phenotypic Study of Arabidopsis thaliana Transformed by Genome Fragment from Populus euphratica

[目的]本研究旨在探索与挖掘胡杨基因组大片段的潜在功能,发掘具有潜在育种价值的胡杨基因簇。[方法]利用已构建的胡杨基因组BIBAC文库,采用花序浸染法,将胡杨基因组大片段78A2D10导入模式植物拟南芥基因组中。采用抗性筛选、分子检测及表型观察等方法鉴定、分析转化型植株。[结果]共获得15株特异表型的转化植株。与野生型相比,转化型植株主侧茎生长受到抑制,莲座叶面积增大近3倍,叶片数量增多,叶边缘皱缩,抽薹推迟约13周,株高增加近32.0 cm,侧茎发育成次生莲座,植株寿命延长约7周。[结论]胡杨基因组片段78A2D10可延长植株营养生长期及植株寿命,据此推测该基因片段可能与营养生长有关。

[ Objective ] The study aims at exploring and excavating the potential function of large genome fragment cloned from Populus euphratica, and finding out the potential gene clusters with breeding value. [ Method ] Based on BIBAC library, the fragment 78A2DlO from the genome of P. euphratica, was inserted into the genome of Arabi- dopsis thaliana by the method of floral-dip. Resistance selection, molecular identification and phenotypic observa- tion were applied to identify and analyze the transgenic plants. [ Result] Fifteen transgenic plants with specific phe- notype were obtained in the research. Compared with the wild plants, the positive plants showed the characters such as inhibited growth of the stems, 3 times rosette leaf area, more leaves, crimping blade edge, delayed bolting with 13 weeks, height increasing by 32.0 cm, developing lateral stems and prolonged lifetime with 7 weeks. [ Conclu- sion] The fragment of 78A2DlO may prolong the vegetative growth and lifetime of plants. The fragment may be re- lated to the vegetative growth of plants.