上调蛋白磷酸酯酶2A水平可显著抑制宫颈癌细胞迁移

Upregulation of Protein Phosphatase 2A Inhibited Cervical Cancer Cells Migration

目的:探讨蛋白磷酸酯酶2A(PP2A)在宫颈癌迁移中的作用。方法:培养Hela宫颈癌细胞,通过药物或质粒转染干预PP2A水平。药物实验中将Hela细胞分为3组,即正常对照组(DMSO处理,n=6),DES组(10nM PP2A激动剂DES处理,n=6)和OA组(10nM PP2A抑制剂OA处理,n=6);质粒转染实验中将Hela细胞分为DsRed-vector组(n=6),DsRed-PP2Ac组(n=6)和DsRed-siPP2Ac组(n=6),按分组要求转染相应质粒。采用划痕实验观察各组不同时间点(药物0h、24h、48h;转染0h、48h)Hela细胞迁移情况并比较组间差异。结果:药物或基因上调PP2A可显著抑制宫颈癌细胞的迁移,而药物或基因下调PP2A水平则明显促进宫颈癌细胞的迁移。结论:PP2A在宫颈癌的发展中起重要的作用,该研究可为宫颈癌的发展提供新的分子机制,亦可为宫颈癌临床药物的开发提供新的靶点。

Objective：To explore the role of protein phosphatase 2 A（PP2 A）in the migration of cervical cancer.Method：Hela cervical cancer cells were cultured and PP2 Alevels were intervened by drug or plasmid transfection.In the drug experiment,Hela cells were divided into three groups,namely normal control group（DMSO treatment,n=6）,DES group（10 nM PP2 Aagonist DES treatment,n=6）and OA group（10 nM PP2 Ainhibitor OA treatment,n=6）.Hela cells were divided into DsRed-vector group（n=6）,DsRed-PP2 Ac group（n=6）and DsRed-siPP2 Ac group（n=6）in plasmid transfection experiments,and the corresponding plasmids were transfected according to grouping requirements.Scratch test was used to observe the migration of Hela cells at different time points（administration0 h,24 h,48 h;transfection 0 h,48 h）and the differences between groups were compared.Result：Upregulation of PP2 A pharmacologically and genetically could inhibit Hela cells migration,however,downregulation of PP2 Apharmacologically and genetically could promote Hela cells migration.Conclusion：PP2 Aplayed an important role in the cervical cancer development,and also would provide new molecular targets for clinical drug research.