归肾丸对卵巢早衰小鼠细胞免疫的影响

Effect of Guishen Pills on cellular immunity in premature ovarian failure mice

目的:探讨归肾丸对卵巢早衰小鼠(POF)细胞免疫的影响。方法:以小鼠透明带3为抗原,皮下多点注射免疫BALB/c雄性小鼠,建立卵巢早衰模型。60只BALB/c小鼠随机分为空白对照组、模型组、泼尼松组、归肾丸高、中、低剂量组。连续给药28 d后,测定各组小鼠的脾脏指数、胸腺指数、卵巢指数、T淋巴细胞增殖能力、T细胞亚群、NK细胞、血清中IFN-γ和IL-2的含量。结果:归肾丸可显著提高小鼠的脾脏指数,增强T细胞的增殖能力,显著增加CD3+T、CD4+T、CD4+T/CD8+T比值、NK细胞百分比及IFN-γ、IL-2的含量(P<0.05),显著降低CD8+T细胞百分比(P<0.05)。结论:归肾丸能够调节机体细胞免疫作用,提高卵巢早衰小鼠的免疫功能。

Objective： To evaluate the protective effect of Guishen Pills on cellular immunity of mice with premature ovarian failure. Methods： BALB/c female mice models of POF were established by multiple sites subcutaneous injection of zona pellucida 3 of mice. Sixty BALB/c mice were randomly divided into blank control group,model group,Prednisone group,high,medium and low dosage groups of Guishen Pills. The mice were treated for sequential 28 days. The spleen index,thymus index,ovary index,the proliferation of T lymphocyte,T lymphocyte subgroup,NK cells and content of IFN-γ,IL-2 of serum were determined. Results： Guishen Pills can significantly increase the spleen index,proliferation ability of T cells,percentage of CD3＋T,CD4＋T,CD4＋T/CD8＋T,NK cells and the content of IFN-γ and IL-2（ P〈0. 05）,significantly decrease percentage of CD8＋T cells. Conclusion： It is indicated that Guishen Pills can regulated body cellular immunity,improve the function of premature ovarian failure mice.