孟鲁司特对过敏性紫癜小鼠炎性因子的影响及其作用机制

Effect and mechanism of montelukast on inflammatory cytokines in Henoch-Schonlein purpura mice

目的:探讨孟鲁司特对过敏性紫癜小鼠炎性因子的影响及其作用机制。方法:采用灌胃给予0.1%麦胶蛋白(溶于6 mmol/L HCl酸化水),尾静脉注射印度墨水(40 mg/kg)封闭网状内皮系统的方法建立过敏性紫癜小鼠模型,采用印度墨水小鼠碳粒廓清法检测网状内皮系统(RES)功能,采用微量聚乙二醇沉淀法测定血清循环免疫复合物(CIC)含量,采用分光光度法测定晚期氧化蛋白产物(AOPPs),采用酶联免疫吸附实验测定IL-1β、IL-6、TNF-α含量。结果:与空白组比较,模型组小鼠RES功能显著降低,血清CIC、AOPPs、IL-1β、IL-6、TNF-α的水平显著升高(P<0.05);与模型组比较,低、中、高浓度孟鲁司特组小鼠RES功能显著提高,血清CIC、AOPPs、IL-1β、IL-6、TNF-α的水平显著降低(P<0.05)。结论:孟鲁司特可以减轻过敏性紫癜小鼠症状,其作用机制与降低氧化应激反应、降低血清AOPPs的含量水平、抑制炎症反应、提高RES功能和增强循环免疫复合物的消除能力有关。

Objective： To investigate the effect and mechanism of montelukast on inflammatory cytokines in Henoch-Schonlein purpura model mice. Methods： Henoch-Schonlein purpura model mice were built by 0. 1% Gliadin（ Dissolved in 6 mmol/L HCl,ig） as antigen and India ink（ 40 mg/kg,ip） to close reticuloendothelial system. The function of reticuloendothelial system was detected by Indian ink mice carbon clearance. Trace polyethylene glycol precipitation was used to determine the content of the circulating immune complex（ CIC） in serum. Spectrophotometry was used to determine the content of advanced oxidation protein products（ AOPPs）. Enzymelinked immunosorbent assay was used to determine the content of IL-1β,IL-6 and TNF-α. Results： Compared with control group,CIC,AOPPs,IL-1β,IL-6 and TNF-α in serum of the mice in model group were significantly increased while the function of RES was significantly reduced（ P〈 0. 05）. Compared with model group,CIC,AOPPs,IL-1β,IL-6 and TNF-α in serum of the mice in different dose group were significantly reduced while the function of RES was significantly increase（ P〈0. 01）. Conclusion： Montelukast can alleviate the symptom of Henoch-Schonlein purpura mice by reducing oxidative stress response,reducing AOPPs levels in serum,inhibiting inflammatory reaction,improving the function of RES,enhancing the ability to eliminate cyclic immune complexes.