养血方与养血解毒方对咪喹莫特诱导的银屑病样小鼠的作用

Effects of Yangxue decoction and Yangxue-Jiedu decoction on imiquimod-induced psoriasis-like mouse model

目的:探讨并比较养血方与养血解毒方对银屑病样小鼠模型皮损的干预作用。方法:将50只BALB/c小鼠随机分为空白对照组、模型组、甲氨蝶呤组、养血方组和养血解毒方组,每组10只,采用背部涂抹咪喹莫特的方式诱导银屑病样模型。动态观察皮损变化并进行银屑病皮损面积和严重程度指数(PASI)评分;通过皮肤水/油测试笔检测小鼠背部皮肤水/油含量;HE染色观察皮损病理改变并检测表皮厚度;免疫组化法检测皮损表皮中增殖细胞核抗原(PCNA)和真皮中CD3+T淋巴细胞表达;real-time PCR法检测皮损中白细胞介素(IL)-17、IL-23和IL-1β的mRNA相对含量。结果:养血方组、养血解毒方组和甲氨蝶呤组背部皮损表现均优于模型组,PASI评分和表皮厚度均低于模型组,皮肤水分/油分含量高于模型组(P<0.05),养血解毒方组背部皮损表现和皮肤水分/油分含量优于养血方组,PASI评分和表皮厚度低于养血方组;养血方组、养血解毒方组和甲氨蝶呤组皮损表皮PCNA表达与真皮CD3+T细胞表达均低于模型组(P<0.05),养血解毒方组表皮PCNA表达与真皮CD3+T细胞表达均低于养血方组;real-time PCR显示养血方组与养血解毒方组IL-17、IL-23和IL-1β的mRNA相对表达量低于模型组(P<0.05),养血解毒方组IL-1β的mRNA相对表达低于养血方组。养血解毒方组与甲氨蝶呤组比较,皮损表现无明显差异,皮肤水/油分含量和对炎症因子的抑制作用优于甲氨蝶呤组。结论:养血方与养血解毒方可通过降低T细胞活化相关因子含量,减轻免疫反应,改善咪喹莫特诱导的银屑病样小鼠皮损;同时养血解毒方效果优于养血方。

AIM： To investigate and compare the effects of Yangxue（ YX） decoction and Yangxue-Jiedu（ YXJD） decoction on psoriasis-like mouse skin lesions. METHODS： BALB/c mice（ n = 50） were randomly divided into control group,model group,methotrexate（ MTX） group,YX group and YXJD group（ 10 mice in each group）. The psoriasis-like mouse model was induced by topical application of imiquimod cream on the back. The skin water/oil test pen was used to detect the water/oil content of the skin in the back of the mice. The pathological changes of the lesions were observed by HE staining and the thickness of the epidermis was measured. The immunohistochemical staining was used to observe the skin lesions,and the mRNA expression levels of interleukin（ IL）-17,IL-23 and IL-1β in skin lesions were detected by real-time PCR. RESULTS： The skin lesions in YX,YXJD and MTX group were better than those in model group,with lower psoriasis area and severity index（ PASI） score and skin thickness. The skin water/oil content in YXJD group was higher than that in model group（ P〈0. 05）. The expression of proliferating cell nuclear antigen（ PCNA） and the positive expression of CD3＋T cells in the skin of YXJD group were lower than those in YX group,and the skin thickness was lower than that in YX group（ P〈0. 05）. The results of real-time PCR showed that relative mRNA expression of IL-17,IL-23 and IL-1β in YX group and YXJD group was lower than that in model group（ P〈0. 05）,and the relative mRNA expression of IL-1β in YXJD group was lower than that in YX group. Administration of YXJD decoction showed better therapeutic effect than MTX. CONCLUSION： YX decoction and YXJD decoction relieve imiquimod-induced skin lesions by reducing immune response. Meanwhile,the effect of YXJD decoction is better than that of YX decoction.