原花青素B1与B2抑制脂多糖诱导的BV-2细胞炎症反应作用的比较

Inhibitory effect of proanthocyanidins B1 and B2 on inflammation of BV-2 cells induced by lipopolysaccharide

目的:比较原花青素二聚体的2种同分异构体——原花青素B1和B2对脂多糖(lipopolysaccharide,LPS)诱导的BV-2细胞炎症反应的抑制作用。方法:体外培养BV-2细胞,MTT法检测原花青素B1与B2对BV-2细胞活力的影响;以1 mg/L的LPS刺激BV-2细胞12 h建立的细胞炎症模型为研究对象,分别采用ELISA法、Transwell趋化实验和Western blot法比较原花青素B1和B2对LPS诱导的BV-2细胞分泌肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素1β(interleukin-1β,IL-1β)、趋化行为及核因子κB(NF-κB)磷酸化的抑制作用。结果:原花青素B1和B2对BV-2细胞没有毒性。与LPS组比较,原花青素B1和B2均可显著降低LPS诱导的TNF-α和IL-1β的释放及BV-2细胞的趋化作用,并抑制NF-κB的磷酸化。结论:原花青素B1和B2均能抑制LPS诱导的BV-2细胞炎症反应,其作用强度无显著差异。

AIM： To investigate the different inhibitory effects of proanthocyanidins B1 and B2,which are isomers,on the inflammatory response of BV-2 cells induced by lipopolysaccharide（ LPS）. METHODS： MTT assay was used to detect the effects of proanthocyanidins B1 and B2 on the viability of BV-2 cells. LPS（ 1 mg/L） was used to promote BV-2 cells to secrete inflammatory factors. ELISA,chemotaxis assay and Western blot were used to detect the influence of proanthocyanidins B1 and B2 on the levels of tumor necrosis factor-α（ TNF-α）,interleukin-1β（ IL-1β）,cell chemotaxis and phosphorylation of NF-κB. RESULTS： Proanthocyanidins B1 and B2 did not show cytotoxicity effect on BV-2 cells. Proanthocyanidin B1 and B2 inhibited the cell chemotaxis,phosphorylation of NF-κB,and releases of TNF-αand IL-1β. CONCLUSION： Proanthocyanidins B1 and B2 inhibit the inflammatory response of BV-2 cells induced by LPS,and their action intensity didn＇t show significant difference.