聚羟基丙烯酸在宫颈组织P16蛋白表达检测中的应用

Application of Polyhydroxy Acrylic Acid in Detection of P16 Protein Expression in Cervical Tissues

目的:观察聚羟基丙烯酸(环保固定液)与10%中性缓冲福尔马林(传统固定液)在免疫组化法检测宫颈组织P16蛋白表达的结果,探讨其替代10%中性缓冲福尔马林的可行性。方法:收集2015年3月—2017年11月于南方医科大学附属中山博爱医院门诊及妇科住院部送检的宫颈组织标本245例,其中包括正常及慢性宫颈炎组52例,低级别鳞状上皮内病变(LSIL)组87例,高级别鳞状上皮内病变(HSIL)组61例,宫颈癌组45例。同一病变部位取材2块,随机分2组,命名为A、B组。A组采用10%中性缓冲福尔马林固定制作切片245张;B组采用聚羟基丙烯酸固定制作切片245张。采用免疫组化法检测宫颈组织P16蛋白表达情况。结果:①生物显微镜下,A、B组切片均背景清晰、阳性着色定位准确、阳性强度可靠、呈色鲜明,无非特异性染色产生、阴性和阳性对照染色结果可靠。②慢性宫颈炎组、LSIL组、HSIL组及宫颈癌组的A组P16蛋白阳性率分别为5.77%、35.63%、80.33%和100.00%,慢性宫颈炎组、LSIL组、HSIL组及宫颈癌组的B组P16蛋白阳性率分别为5.77%、33.33%、81.97%和100.00%。A、B组间对应的各级宫颈病变,P16蛋白表达阳性率差异均无统计学意义(均P>0.05)。结论:用聚羟基丙烯酸替代10%中性缓冲福尔马林对免疫组化法检测宫颈组织P16蛋白表达阳性率无明显影响,聚羟基丙烯酸作为固定液在免疫组化法检测宫颈组织P16蛋白表达中有一定的使用价值。

Objective：To observe the expression of P16 protein in cervix tissue by immunohistochemical method and10% neutral buffer Faure Marin（traditional stationary liquid）, and explore the feasibility of replacing it with 10% neutral buffer Faure Marin. Methods：245 specimens of cervical tissue were collected from the outpatient department and the department of gynaecologic inpatient department of Zhongshan BOAI Hospital Affiliated to Southern Medical University from March 2015 to November 2017. There were 52 cases of normal and chronic cervicitis, 87 cases in group LSIL, 61 in group HSIL and 45 in cervical cancer group. 2 pieces of the same lesion were collected and randomly divided into two groups, which were named group A and B. In group A, 245 sections were made by 10% neutral buffered formalin, and 245 sections were made by polyhydroxy acrylic acid in group B. Immunohistochemical method was used to detect the expression of P16 protein in cervical tissue. Results：①Under the biological microscope, the two groups of A and B slices had clear background, positive collocation,accurate location, strong positive intensity, bright and fresh color, and no specific staining, negative and positive staining results were reliable.② In group A, the positive rate of p16 protein in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group were 5.77%, 35.63%, 80.33% and 100.00%, respectively. The positive rate of p16 protein in B group was 5.77%,33.33%, 81.97% and 100.00% respectively in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group.There was no significant difference in the positive rate of P16 protein expression between A and B groups（P 0.05）.Conclusions：Using polyhydroxyacrylic acid instead of 10% neutral buffer Faure Marin has no significant difference on the positive rate of P16 protein expression in cervical tissue by immunohistochemical method. Polyhydroxyacrylic acid as a stationary liquid has certain value in the detection of P16 protein expression in cervical tissue by immunohistochemical method.