高效复合助剂对除草剂草甘膦的作用效果

Effect of Different Composite Auxiliaries on the Osmosis of Glyphosate through the Cuticle of Apple

[目的]研究高效复合助剂对除草剂草甘膦的作用效果。[方法]使用苹果离体角质膜为试验材料,采用模拟渗透法,使用紫外分光光度计检测草甘膦的渗透量,研究不同复合型农药助剂对草甘膦渗透的促进作用。选择磷酸三丁酯(TBP)、癸二酸二乙酯(DES)、癸二酸二丁酯(DBS)3种助剂不同配方的复合型助剂作为试验试剂,不加助剂作为空白对照(CK),比较在不同时间、不同温度下不同浓度草甘膦在苹果角质膜上的渗透量。[结果]无论在何种条件下,随着草甘膦浓度的增加,草甘膦的渗透量均增加。在20℃、72 h条件下,滴加4.0μg/m L草甘膦的样品与滴加2.0μg/m L草甘膦的样品相比,其OD240提高了27%。2种复合型助剂均对草甘膦在离体苹果角质膜上的渗透有促进作用,复合型助剂I的促进作用高于复合型助剂Ⅱ,在草甘膦浓度2.0μg/m L、湿度100%条件下,随着温度的升高,草甘膦的渗透量有不同程度的增加,在温度25℃、48 h时,2种复合型助剂的促进作用达到最好,其OD240比对照组提高了26.1%,复合型助剂II则提高了14.8%。在72 h时,CK、复合型助剂Ⅰ、复合型助剂Ⅱ草甘膦渗透量的OD240均达到最大。[结论]复合型助剂I对除草剂草甘膦的促进效果最好。

[Objective]To study effect of different composite auxiliaries on the osmosis of glyphosate through the cuticle of apple.[Method]The isolated apple keratinocyte membranes were used as an experimental material to simulate the penetration and absorption of glyphosate,and the penetration of glyphosate on the membrane was measured by using an ultraviolet spectrophotometer to study the effect of different types of composite auxiliaries on the penetration of glyphosate. Tributyl phosphonate（TBP）,diethyl sebacate（DES）,and dibutyl sebacate（DBS） were used as experimental reagents with different ratios,and no adjuvant as the blank control（CK）,and the penetration of different concentrations of glyphosate on the keratinocytes of apples was compared.[Result] No matter what conditions,with the concentration of glyphosate going up,the osmolality of glyphosate increased.At 20 ℃,72 h,the OD value of the glyphosate sample at 4.0 g/m L glyphosate had 27% increase in the OD value of the glyphosate sample at 2.0 μg/m L.Composite auxiliaries I and composite auxiliaries II both had obvious promoting effect,composite auxiliaries I promoting effect was higher than composite auxiliaries II,at glyphosate sample at 2.0 μg/m L,100% humidity,as the temperature going up,the OD value of the glyphosate sample had different degrees of increase.At 25 ℃,48 h,composite auxiliaries I and composite auxiliaries II both had the most significant effect on the osmotic effect of glyphosate,compared with the blank control group of the OD value,composite auxiliaries I had 26.1% increased,however composite auxiliaries II had 14.8% increased. At 72 h,the OD value of the glyphosate sample all had the maximum value.[Conclusion]The effect of composite auxiliaries I on glyphosate is best.