β-CD包络肽的介孔硅纳米塞的构建与控释性能研究

Construction and Controlled Release Properties of Mesoporous Silica Nanoplugs with β-CD Envelope Peptide

以MCM-41型介孔硅为载体,在外表面修饰了二硫键(S-S),以二硫键为连接剂接枝了两个氨基酸(半胱氨酸和色氨酸)形成二肽,二肽末端的色氨酸含有疏水吲哚结构,以β-环糊精(β-CD)的疏水内部空腔将二肽包络成为"纳米塞",实现介孔硅孔道的封堵。以亚甲基蓝(MB)为模拟药物进行了载药和谷胱甘肽(GSH)刺激响应释放实验,结果表明,载MB的介孔硅在无GSH刺激时不会释放出MB,而当在10mmol/L GSH环境中时,二硫键断裂使得"纳米塞"脱落从而快速释放负载的大量MB。

Using MCM-41 type mesoporous silica as a carrier,disulfide bond（ S-S） have been modified on its outer surface.Two amino acids（ cysteine and tryptophan） were grafted to form a dipeptide using a disulfide bond as the linker. The tryptophan at the end of the dipeptide contains a hydrophobic quinone structure,and the dipeptide is enveloped in a hydrophobic internal cavity of β-cyclodextrin（ β-CD） to form a ＂nano-plug＂,thereby realizing the sealing of the mesoporous silica channel.Methylene blue（ MB） was used as a mimic drug to carry out the drug release and glutathione（ GSH） stimulated response release experiment. Mesoporous silica loaded with MB does not release MB without GSH stimulation,while under the stimulation of10 mmol/L GSH,the disulfide bond was cut off and rapidly released a large number of MB loaded.