载PTH-Fc/PLCL/SF静电纺丝屏障膜的构建与性能研究

Fabrication and Characterization of Fibrous Barrier Mat of Poly(L-lactide-co-caprolactone)/silk Fibroin Loaded with PTH-Fc

目的:利用静电纺丝技术制备携载PTH-Fc的PLCL/SF三维纤维纳米屏障膜,并研究其生物相容性、生物降解能力、机械强度和调节细胞活动的能力。方法:制备PTH-Fc/PLCL/SF屏障膜,并与PLCL/SF屏障膜对照,扫描电镜研究其降解前后微观形貌,拉伸实验研究其机械强度,MTT测试与细胞粘附性实验研究屏障膜的生物相容性,茜素红染色研究携载PTH-Fc缓释是否可以影响细胞成骨分化。结果:PTH-Fc/PLCL/SF屏障膜纳米纤维丝均一光滑。PTH-Fc/PLCL/SF与PLCL/SF屏障膜断裂强度为(5.56±0.18)MPa、(5.42±0.03)MPa,两者无统计学意义。PTH-Fc/PLCL/SF生物降解能力优异,PTH-Fc/PLCL/SF与PLCL/SF屏障膜均可以促进细胞增殖(P<0.05),两者之间无统计学意义,但PTH-Fc/PLCL/SF能促进细胞分化。结论:PTH-Fc/PLCL/SF屏障膜有良好的机械强度、适宜的降解能力,能促进BMMSCs细胞增殖与分化。

Objective： To fabricate fibrous barrier mat of poly（L-lactide-co-caprolactone）/silk fibroin loaded with PTH-Fc via electrospinning technique, and to study its biocompatibility, biodegradability, mechanical strength, and ability to regulate appropriate cellular activities. Methods： Electrospun fibrous poly（L-lactide-co-caprolactone）/silk fibroin loaded with PTH-Fc （PLCL/SF/PTH-Fc） or without （PLCL/SF） were prepared and compared. Apparent morphology before and after degradation were observed by scanning electron microscope （SEM）. Tensile property was tested by custom mechanical testing device. The biocompatibility of PLCL/SF/PTH-Fc was evaluated by MTT and the cell adhesion assay. The osteogenic potential of the cells cultured with PLCL/SF/PTH-Fc was investigated by alizarin red stain. Results： The nanofibers of PLCL/SF/PTH-FC were uniform and smooth. The breaking strengths of PLCL/SF/PTH-Fc and PLCL/SF were （5.56±0.18） MPa and （5.42±0.03） MPa, respectively （P〉0.05）. PLCL/SF/PTH-Fc mats could degrade quickly, promote the cell proliferation, and induce the cell differentiation. Conclution： Electrospun fibrous PLCL/SF/PTH-Fc has good mechanical property and biodegradability. It can promote cell proliferation and cell differentiation.