B7-H4在结核性胸膜炎患者mDC中的表达与调节性T细胞的相关性研究

Expression of B7-H4 in mDC in patients with tuberculous exudative pleurisy and its correlation with regulatory T cells

目的 探讨共刺激分子B7-H4在结核性渗出性胸膜炎患者血清和胸腔积液的表达及其与调节性T细胞的相关性.方法 选择2016年8月至2017年8月广东医科大学附属厚街医院呼吸内科结核性渗出性胸膜炎30例,健康体检者15例,采用S-P免疫组织化学染色检测（流式细胞术检测）B7-H4在结核性渗出性胸膜炎患者及健康对照者外周血以及结核性渗出性胸膜炎患者胸水mDC中的表达情况;并分析结核性渗出性胸膜炎患者血清和胸腔积液Treg细胞的浸润情况.结果 结核性渗出性胸膜炎患者外周血B7-H4的mDC比例显著高于健康对照组,并且结核渗出性胸膜炎患者胸水中的比例则显著高于其外周血（经过治疗后结核渗出性胸膜炎患者外周血B7-H4的mDC比例较治疗前下降明显,但仍高于对照组外周血的比例）.30例结核性渗出性胸膜炎患者中B7-H4的表达与Treg细胞数量正相关,治疗后B7-H4在患者外周血来源的mDC中的表达水平显著降低.结论 B7-H4在结核渗出性胸膜炎患者胸水及外周血中高表达,而且与Treg细胞数量正相关,可反应机体受MTB感染后的免疫功能状态,其比例变化亦可影响结核渗出性胸膜炎的发展进程,可能成为结核性胸膜炎诊断、了解其免疫状态的重要依据.

Objective To investigate the expression of costimulatory molecule B7-H4 in serum and pleural effusion of patients with tuberculous exudative pleurisy and its correlation with regulatory T cells.Methods 30 cases of tuberculous exudative pleurisy treated at the Department of Respiratory Medicine,Houjie Hospital from August 2016 to August 2017 were selected.15 healthy cases taking physical examination were examined.S-P immunohistochemical staining （flow cytometry） was used to detect B7-H4 in the patients＇ mDC and the healthy examinees＇ peripheral blood.The infiltration of Treg cells in serum and hydrothorax of the patients was analyzed.Results The ratio of B7-H4 in mDC was significantly higher in the patients than in the healthy cases,and was significantly higher in the hydrothorax than in the peripheral blood of the patients.After the treatment,the B7-H4 in mDC decreased in the patients＇ peripheral blood,but was still higher in the healthy cases＇.In the patients,the expression of B7-H4 positively correlated with Treg cell number.Conclusions B7-H4 highly expresses in the hydrothorax and peripheral blood of patients with tubercular exudative pleurisy,positively correlates with the number of Treg cells,and can reflect the immune function state after MTB infection.