异育银鲫源嗜水气单胞菌对磺胺类耐药性分析

Drug Resistance of Aeromonas hydrophila Isolated from Allogyogenetic Silver Crucian Carp Carassius auratus gibelio to Sulfonamides

为了解异育银鲫源嗜水气单胞菌对磺胺类药物的敏感性和磺胺类耐药基因的携带情况。采用纸片扩散法和微量肉汤稀释法测定了36株受试菌株对常见4种磺胺类药物的敏感性;应用PCR法检测菌株染色体DNA和质粒DNA磺胺类Sul1、Sul2和Sul3耐药基因。试验结果表明,试验菌株对磺胺嘧啶、磺胺二甲嘧啶、磺胺异噁唑、磺胺甲噁唑的耐药率分别为83.34%、72.22%、66.66%、69.45%;36株嗜水气单胞菌的染色体和质粒中均检测到Sul1和Sul2基因,Sul1基因在染色体和质粒中的检出率分别为30.56%和25.64%,Sul2基因在染色体和质粒中的检出率分别为83.33%和91.67%,Sul3基因在染色体和质粒中均未检出。异育银鲫源嗜水气单胞菌对磺胺类药物的耐药性不容乐观,其耐药性与Sul2和Sul1基因有较大相关性。

The sensitivity of 36 strains of Aeromonas hydrophilaisolated from allogyogenetic silver crucian carp Carassius auratus gibelio to four common sulfonamides was studied by disk diffusion method and broth dilution method and the sulfa drug resistance genes about Sul1,Sul2 and Sul3 were detected by a PCR method from chromosome of DNA and plasmid DNA in order to understand the sulfa drug sensitivity and sulfa drug resistance genes.It was found that the test strains had drug resistance rate of 83.34% to sulfadiazine,72.22% to sulfamethazine,and 66.66% to sulfamethoxazole,and 69.45% to sulfamethoxazole.A total of 36 strains of A.hydrophila chromosomes and plasmids were detected in Sul1 and Sul2 genes,with Sul1 gene detection rate of 30.56%in the chromosome and 25.64% in plasmid,Sul2 gene 83.33%in the chromosome and 91.67% in plasmid and without detection Sul3 gene in chromosome and plasmid.It is not optimistic about the sulfa drugs resistance of A.hydrophilaisolated from allogyogenetic silver crucian carp,and its resistance is closely related to genes Sul2 and Sul1.