溴氰菊酯对中华绒螯蟹血细胞DNA的损伤

Effects of Deltamethrin on DNA Damage in Hemocytes of Chinese Mitten Handed Crab,Eriocheir sinensis

为研究溴氰菊酯对中华绒螯蟹的遗传毒性作用,将中华绒螯蟹分为5组进行半静水毒性试验,分别为0μg/L的空白对照组,只添加丙酮的溶剂对照组,以及0.10、0.20、0.40μg/L的溴氰菊酯质量浓度试验组。通过彗星检测的方法,分别测定溴氰菊酯暴露1、2、4、8、12d后中华绒螯蟹血细胞拖尾率及Olive尾距(OTM值)。结果显示,溶剂组在各时间点拖尾率及Olive尾距均同对照组无显著差异(P>0.05),表明溶剂丙酮并非造成中华绒螯蟹血细胞DNA损伤的原因。溴氰菊酯对中华绒螯蟹血细胞有较强的DNA损伤作用,1d后0.20μg/L和0.40μg/L组拖尾率和Olive尾距均显著高于对照组(P<0.05),此后3个质量浓度组在各时间点均极显著高于对照组(P<0.01),至12d后仍未恢复。拖尾率和Olive尾距随着暴露时间增加而上升,呈现明显的时间效应和剂量效应,表明其可以作为评估溴氰菊酯对中华绒螯蟹遗传毒性的敏感指标。

In order to investigate genotoxicity of deltamethrin in Chinese mitten handed crab,Eriocheir sinensis,a semi-static toxicity test was conducted in this study.Crabs were divided into 5 groups including0μg/L deltamethrin as blank control,addition of acetone only as solvent control and 0.10,0.20 and 0.40μg/L as treatment groups.Comet ratios and Olive Tail Moments（OTM）were measured at 1 d,2 d,4 d,8 dand 12 dafter comet assay.The results showed that there was no significant difference between solvant control and blank control,indicating that acetone was not the reason for DNA damage in hemocytes of the crab.Deltamethrin had so strong effects on DNA damage in hemocytes that the comet ratio and OTM in0.20 and 0.40μg/L groups were higher than that in blank control significantly after 1 dexposure,and then was significantly higher than that in blank control at each time point without a recovering till 12 d.In additon,the comet ratio and OTM presented time-response and dose-response obviously during this expriment,which reveals that they could be used as sensitive indicators in genotoxicity assessment of deltamethrin.