正交试验法优化车前子中槲皮素提取工艺

Optimization of extraction process of quercetin from Semen Plantaginis by orthogonal test

目的研究从车前子中提取槲皮素的最佳条件。方法以车前子作为提取槲皮素的原料,用高效液相色谱法(HPLC)进行提取条件的单因素试验,研究的因素包括超声功率、提取时间、甲醇比例、料液比例,以此为依据进行L9(34)正交试验,寻求最佳提取条件。结果研究表明各因素对槲皮素提取效果的影响大小依次为料液比>超声功率>甲醇比例>提取时间,从车前子提取槲皮素的最佳条件是选择40%甲醇水溶液为提取剂,以1∶40(g/ml)的料液比,在200 W的超声功率下超声40min进行提取。结论在优化后的提取条件下,槲皮素提取率达到0.79%,能简便、稳定且高效地从车前子中提取槲皮素。

Objective To search for the most effective condition of extracting quercetin from Semen Plantaginis.Methods Single factor experiments of extracting quercetin from Litchi Semen Plantaginis were carried out by HPLC.Ultrasonic power,extracting time,methanol percentage and solid/liquid ratio were selected as critical factors.Orthogonal array design L9（34）was carried out to search for the most efficient condition to extract quercetin.Results The influential factors for quercetin extraction were as follows,solid/liquid ratioultrasonic powermethanol percentageextracting time.The most optimal condition was to use 40% methanol to extract for 40 min with 200 W ultrasonic power and a solid/liquid ratio of 1∶40（g/ml）.Conclusion Under this optimal condition,the extraction yield of quercetin reached 0.79%.This condition offered a simple,stable and effective process to extract Quercetin from Semen Plantaginis.