吉他霉素发酵培养基原材料替代

Substitution of Kitomycin Fermentation Medium Raw Materials

探讨了吉他霉素发酵培养基中各氮源对发酵效价的影响,同时利用各有机氮源替代配方中的丝素粉以达到提高效价的目的,结果表明无机氮源硫酸铵按0.75∶1等量比替代配方中的丝素粉,可提高吉他霉素效价5.1%,同时降低生产成本,原材料成本每月节约26.7万元。

The effects of various nitrogen sources on the fermentation titers in the kitasamycin fermentation medium were discussed. At the same time,the use of various organic nitrogen sources was substituted for the silk fibroin powder in the formula to achieve the purpose of increasing potency. The results showed that the inorganic nitrogen source was ammonium sulfate. 0. 75∶ 1 is equivalent to the silk fibroin powder in the alternative formula,which can increase the guitaric acid titer by 5. 1% while reducing the production cost. The cost of raw materials can save 267,000 yuan per month.