响应面在加拿大一枝黄花挥发油提取中的应用

Application of Response Surface Design on Extraction of Volatile Oil from Solidago Canadensis L

用响应面优化加拿大一枝黄花挥发油的提取。[方法]:超声提取挥发油,依据Box-Behnken试验设计原理和quadratic模型,以超声时间:28.45,40,51.55min;提取温度:36.34,45,53.66℃;pH值:5,7,9为影响值,采用三因素三水平响应面分析法,以挥发油萃取率为响应值进行方差分析,获取多元二次线性回归方程。[结果]:响应面试验方差分析表明,超声时间显著影响挥发油萃取率(P=0.0042<0.05),而提取温度和pH值对萃取率影响不显著。最佳萃取条件为超声时间为43min,提取温度为37℃,pH值为7.45。萃取率模型预测最大值为71.97%。[结论]:在最优条件下再次实验得到的试验值为71.963%,此实验合理可行。

Objective： To optimize the extraction of Solidago canadensis L. volatile oil yield with the response surface. Methods： Box behnken design was utilized to maximize the volatile oil yield , and ultrasonic extraction time, extracting temperature and pH was investigated. Under a 3 factor ＆ 3 level responsesurface system, the ultrasonic extraction time was fixed at 28.45, 40 and 51.55min; the extracting timewas fixed at 36.34, 45 and 53.66 min; the pH was fixed at 5, 7 and 9, respectively. The final yield, which was investigated as response value was analyzed by variance analysis, and a two times multivariate linearregression equation was acquired. Result： Response surface displays that the ultrasonic extraction time wasutilized to maximize the volatile oil yield（P〈0.05） , while extracting temperature and pH made no difference（P〉0.05）. The best condition： the ultrasonic extraction time was 43min, extracting temperature was37℃ , pH was 7.45,and forecasted the maximumvalue was 71.97% of the yield model. Conclusion：In thebest condition, the maximum value was 71. 963% was examined, so the experiment is feasible.