BNP信号通路在眶下神经损伤大鼠模型中的研究

BNP signaling pathway in a rat model of infraorbital nerve injury

目的通过构建大鼠三叉神经痛动物模型,探究眶下神经慢性压迫对大鼠三叉神经节内BNP受体NPR-A表达及cGMP含量的影响。方法通过大鼠眶下神经慢性压迫损伤构建三叉神经痛动物模型,采用Von Frey hairs检测模型大鼠触须垫区域机械刺激疼痛阈值,应用Western blot技术检测NPR-A在模型大鼠三叉神经节内的表达,ELISA技术检测模型大鼠三叉神经节内cGMP的含量。结果与对照组大鼠比较,眶下神经慢性压迫损伤组(ION-CCI组)大鼠在术后第1周~第3周出现术侧面部机械刺激疼痛阈值的降低,差异有统计学意义(P<0.001)。两组模型大鼠三叉神经节中均有NPR-A和cGMP蛋白表达,相比于对照组大鼠,IONCCI组大鼠三叉神经节中NPR-A和cGMP蛋白表达均显著增加(P<0.001)。结论 BNP信号通路可能参与大鼠三叉神经痛的发生和发展。

Objective To establish the trigemina neuropathic（ TN） pain model rats by infraorbital nerve chronic constriction injury（ ION-CCI） and investigate the effects of the infraorbital nerve（ ION） after chronic constriction injury（ CCI） on the expression of BNP receptor NPR-A and cGMP content in trigeminal ganglion（ TG） of model rats. Methods TN model rats were produced by ION-CCI. Von Frey hairs were used for the mechanical stimulation test on the ipsilateral whisker pad of rats. Western blot was used to detected the expression of NPR-A in TG.The content of cGMP in TG was detected by ELISA. Results From the 1 st week to 3 rd week after ION-CCI,the rats showed significantly increased responses to mechanical stimulation in the territory of the ligated nerve,which had a statistically significant difference（ P〈0. 001） compared with that in the control group. Both NPR-A and cGMP were expressed in TG of the two groups. The expression level of NPR-A and cGMP in the TG of ION-CCI group were significantly enhanced compared with the control group（ P〈0. 001）. Conclusion BNP signal pathway may be participate in the development of TN.