成骨细胞特异性识别多肽对人成骨细胞黏附和迁移作用的实验研究

The experimental study of effect of the peptide that specifically bind to osteoblasts on attachment and migration of human calvarial osteoblasts in vitro

目的探讨成骨细胞特异性识别多肽对人颅骨成骨细胞黏附和迁移作用的影响。方法常规培养人颅骨成骨细胞,分为10^(-4)、10^(-5)、10^(-6)、10^(-7)、10^(-8)mol/L 5个实验组及对照组,应用黏附实验、划痕实验及Transwell迁移小室实验检测不同浓度的成骨细胞特异性识别多肽对人成骨细胞黏附、迁移作用的影响。结果成骨细胞特异性识别多肽在10^(-8)~10^(-4)mol/L浓度范围内能够促进人颅骨成骨细胞迁移与黏附,其中10^(-5)mol/L浓度促进作用最为显著。结论成骨细胞特异性识别多肽能够促进成骨细胞黏附及迁移。

Objective To investigate the effect of the peptide that specifically bind to osteoblasts on the human calvarial osteoblasts attachment and migration in vitro. Methods Human calvarial osteoblasts were routinely cultured which were divided into 6 groups including experimental groups containing the peptide at various concentrations（10^（-4）,10^（-5）,10^（-6）,10^（-7）,10^（-8） mol/L） and the control group. The migration activity was examined by scratch test and Transwell chamber migration test on different time. Finally,experimental data were statistically analyzed.Results The peptide that specifically binds to osteoblasts in the concentrations of 10^（-8） mol/L to 10^（-4） mol/L could promote the migration and attachment of human calvarial osteoblasts in vitro,and the best concentration was 10^（-5） mol/L. Conculsion The peptide that specifically binds to osteoblasts can promote the migration and attachment of human calvarial osteoblasts in vitro.