恒河猴急性酒精暴露颞叶静息态脑功能改变9.4T MRI初步研究

A preliminary study of 9. 4T MRI on the changes of the temporal resting state of the brain in the acute alcohol exposure of rhesus monkey

目的用9.4T静息态功能磁共振(f MRI)基于分数低频振荡幅度(f ALFF)算法分析恒河猴急性酒精暴露颞叶脑功能变化。方法先后对5只健康雄性恒河猴行静脉注射酒精前(自身对照组)和静脉注射酒精10 min后(酒精组)9.4T静息态f MRI扫描,经f ALFF算法分析并比较两组颞叶f ALFF值,寻找差异脑区。结果酒精组颞叶f ALFF值低于自身对照组的脑区为左侧颞上回、左侧颞中回、左侧颞下回(P<0.01);酒精组颞叶f ALFF值高于自身对照组的脑区为右侧颞上回、右侧颞叶海马旁回、左侧颞下回(P<0.01)。结论利用9.4T rs-f MRI进行恒河猴急性酒精暴露颞叶脑功能改变研究是安全可行的;恒河猴急性酒精暴露颞叶脑功能改变明显,右侧增强,左侧以减低为主,其中左侧颞下回呈混合性改变。

Objective To analyze the functional changes of temporal lobe of acute alcohol exposure in rhesus monkeys by rs-f MRI at 9. 4 T based on fractional low frequency oscillation amplitude（ f ALFF） algorithm. Methods Five healthy male rhesus monkeys were anesthetized with 9. 4 T rs-f MRI before intravenous injection of alcohol（ selfcontrol group） and intravenous alcohol 10 minutes（ alcohol group）. The f ALFF algorithm was used to analyze and compare the f ALFF values of the temporal lobe of the two groups to find the different brain regions. Results In the alcohol group,the f ALFF of the temporal lobe was lower than that of the control group（ left superior temporal gyrus,left middle temporal gyrus,left inferior temporal gyrus）（ P〈0. 01）,the f ALFF of the temporal lobe was higher than that of the control group（ right superior temporal gyrus,right temporal gyrus parahippocampal gyrus,left inferior temporal gyrus）（ P〈0. 01）. Conclusion It is safe and feasible to use 9. 4 T rs-f MRI to study the changes of temporal lobe brain function after acute alcohol exposure in rhesus monkeys. The temporal lobe brain function of acute alcohol exposure of rhesus monkeys is obvious. The right side is enhanced and the left side is reduced mainly,and the left inferior temporal gyrus shows a mixed change.