RP-HPLC法测定不同产地杉寄生药材中落新妇苷和芦丁的含量

Content Determination of Astilbin and Rutin in Macrosolen cochinchinensis from Different Producing Areas by RP-HPLC

目的:建立同时测定杉寄生药材中2种黄酮类成分落新妇苷和芦丁含量的反相高效液相色谱(RP-HPLC)法,并比较不同产地药材中2种成分的含量。方法:色谱柱为SHISEIDO-SPOLAR C18,流动相为乙腈-0.1%磷酸溶液(14∶86,V/V),流速为1.0m L/min,检测波长为291 nm,柱温为35℃,进样量为10μL;对来源于广西和广东6个市/县的杉寄生药材进行含量测定及比较分析。结果:落新妇苷和芦丁的检测质量浓度线性范围分别为22~440、12~240μg/m L(r分别为0.999 6、0.999 1),平均加样回收率分别为99.44%、99.96%(RSD分别为1.38%、1.50%,n=9);杉寄生中落新妇苷含量在各省/区及各市/县之间均相似(0.862 5~0.901 1 mg/g),芦丁含量在同一省/区的市/县间相似,但在2个省/区之间差异较大(平均值分别为0.431 3、0.214 9 mg/g)。结论:本法简便、准确、稳定、重现性好,可用于杉寄生药材的质量控制。

OBJECTIVE：To establish RP-HPLC method for simultaneous determination of astilbin and rutin in Macrosolen cochinchinensis,and to compare the contents of 2 components in medicinal material from different producing areas. METHODS：The determination was performed on SHISEIDO-SPOLAR C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid（14∶86,V/V） at the flow rate of 1.0 m L/min. The detection wavelength was set at 291 nm,and column temperature was35 ℃,the sampling volume is 10 μL.Content determination and comparative analysis of M. cochinchinensis from 6 counties of Guangxi and Guangdong were conducted. RESULTS：The linear range of astilbin and rutin were 22-440 μg/m L（r=0.999 6）and12-240 μg/m L（r=0.999 1）;average recoveries were 99.44%（RSD=1.38%,n=9）and 99.96%（RSD=1.50%,n=9）. The contents of astilbin in M. cochinchinensis were similar between provinces/autonomous region or among cities/counties（0.862 5-0.901 1 mg/g）;the contents of rutin were similar among cities/counties in same provinces/autonomous region but there was a great difference between provinces/autonomous region（average values were 0.431 3,0.214 9 mg/g）. CONCLUSIONS：This method is simple,accurate,stable and reproducible for the quality control of M. cochinchinensis.