汉防己甲素作用胃癌细胞系HGC-27后差异表达miRNAs筛选及生物信息学分析

Screening and bioinformatics analysis of differentially expressed miRNAs in HGC-27 gastric cancer cells treated by tetrandrine

目的筛选汉防己甲素(Tet)处理胃癌细胞系HGC-27后差异表达的微小RNA(miRNAs),并进行生物信息学分析。方法应用高通量测序技术对HGC-27细胞和Tet处理后的HGC-27细胞进行miRNAs差异表达谱分析;结合文献报道确定4个与肿瘤相关的miRNAs,对其进行靶基因预测及信号转导通路的分析。结果经Tet处理后,miR-7641和miR-149-3p的表达水平明显上调,miR-500a-5p和miR-122-5p的表达水平明显下调;生物信息学方法分析结果显示miR-7641、miR-149-3p、miR-500a-5p、miR-122-5p及其靶基因参与细胞迁移、细胞周期和凋亡等通路。结论 Tet可能通过调控miR-7641、miR-149-3p、miR-500a-5p、miR-122-5p的表达参与胃癌的发生发展。

Objective To screen differentially expressed microRNAs（miRNAs） in HGC-27 gastric cancer cells treated by tetrandrine（Tet） and perform bioinformatics analysis. Methods High-throughput sequencing technology was used to analyze the differential expression profiles of miRNAs in HGC-27 cells and Tet-treated HGC-27 cells. Four tumor-associated miRNAs had been identified, and the target genes were predicted and their signal transduction pathways were analyzed. Results After Tet treatment, the expression levels of miR-7641 and miR-149-3 p were significantly up-regulated,and the expression levels of miR-500 a-5 p and miR-122-5 p were down-regulated. Bioinformatics analysis showed that the target genes of miR-7641,miR-149-3 p, miR-500 a-5 p and miR-122-5 p were involved in cell migration, cell cycle, and apoptosis pathways. Conclusion Tet may participate in the development of gastric cancer by mediating the expression levels of miR-7641,miR-149-3 p,miR-500 a-5 p and miR-122-5 p.