miR-615-3p对胃癌细胞增殖和凋亡的影响

Effect of miR-615-3p on the proliferation and apoptosis of gastric cancer cells

目的研究miR-615-3p对胃癌细胞HGC-27增殖与凋亡的影响。方法脂质体转染miR-615-3p抑制剂(miR-615-3p inhibitor)和inhibitor阴性对照进入胃癌细胞HGC-27中,MTT法检测miR-615-3p inhibitor对胃癌细胞HGC-27增殖情况的影响,Annexin V-FITC细胞凋亡检测试剂盒检测转染后胃癌细胞HGC-27凋亡的变化。结果 MTT结果显示:转染miR-615-3p inhibitor 48 h、72 h时,A490分别为0.527±0.011、0.787±0.020,inhibitor阴性对照A490分别为0.583±0.022、0.906±0.038,与inhibitor阴性对照比较,差异有统计学意义(P<0.05),胃癌细胞HGC-27的增殖变慢;转染96 h时,A490值为0.857±0.017,inhibitor阴性对照A490为0.951±0.016,与inhibitor阴性对照对比,细胞活性抑制得更明显(P<0.01)。流式细胞术结果显示:miR-615-3p inhibitor在转染48 h、72 h后,细胞凋亡率达(15.403±0.209)%、(20.130±0.239)%,inhibitor阴性对照为(12.655±0.112)%、(14.810±0.643)%,与inhibitor阴性对照比较,胃癌细胞HGC-27的凋亡率显著增高,凋亡数目明显高于inhibitor阴性对照(P<0.01)。结论降低miR-615-3p在HGC-27细胞的表达水平,能有效抑制HGC-27细胞的增殖能力并促进其凋亡。

Objective To investigate the effect of miR-615-3 p on the proliferation and apoptosis of gastric cancer HGC-27 cells. Methods The miRNA-615-3 p inhibitor（miR-615-3 p inhibitor, miR-615-3 p I） or inhibitor negative control（inhibitor negative control, NC） was transfected into HGC-27 cells by lipofectamine. Then the effect of miRNA-615-3 p inhibitor on the proliferation of HGC-27 cells was detected by MTT assay, and the cell apoptosis were analyzed by Annexin V-FITC staining. Results MTT assay showed that the A490 values were 0.527 ± 0. 011 and 0. 787 ± 0. 020 after transfected with miR-615-3 p I for48 h and 72 h,and the A490 values of inhibitor negative control were up to 0.583± 0.022 and 0.906± 0.038,respectively. Compared with the NC group,the difference in miR-615-3 p I group was statistically significant（P〈 0.05）. The growth of HGC-27 cells was reduced after transfected with miR-615-3 p I. When transfection for 96 h, the A490 values were 0. 857 ± 0. 017 in miR-615-3 p I group, and 0. 951 ± 0. 016 in the NC group（P〈 0.01）. Flow cytometry showed that the apoptosis of HGC-27 cells were up to（15.403± 0.209） % and（20.130± 0.239） % after transfected with miR-615-3 p I for 48 h and 72 h,whereas the apoptosis of HGC-27 cells were（12.655± 0.112） % and（14.810± 0.643） % in the NC group. Compared with the NC group,the apoptosis rate and apoptosis number of HGC-27 cells were significantly increased in miR-615-3 p I group（P〈 0. 01）. Conclusion Inhibition of MiR-615-3 p level in HGC-27 cells can reduce the cell growth and promote cell apoptosis.