Th17细胞与Treg细胞及相关细胞因子在SLE患者外周血中的表达及意义

Expressions and Significance of Th17 Treg Cell and Related Cytokines in Peripheral Blood in Patients with SLE

目的:探讨Th17细胞与Treg细胞及相关细胞因子在系统性红斑狼疮(SLE)患者外周血中的表达及意义。方法:选取我院2016年5月至2017年4月收治的SLE患者65例,依据SLE疾病活动指数(SLEDAI)分为活动组33例和非活动组32例,另选取同期健康体检者25例为对照组。采集受试者空腹外周静脉血,采用散射速率免疫比浊法检测血清Ig A、Ig G、Ig M、C3、C4水平,采用流式细胞仪测定Th17细胞和Treg细胞的表达,采用ELISA法检测白细胞介素-6(IL-6)、白细胞介素-17(IL-17)、白细胞介素-23(IL-23)、转化生长因子-β(TGF-β)的表达。结果:SLE活动组患者IgA、IgG、IgM高于非活动组和对照组,C3、C4低于非活动组和对照组,SLE非活动组患者IgA、IgG高于对照组;SLE活动组患者Th17细胞、Th17/Treg高于非活动组和对照组,Treg细胞低于非活动组和对照组,SLE非活动组患者Th17细胞、Th17/Treg高于对照组,Treg细胞低于对照组;SLE活动组患者IL-6、IL-17、IL-23高于非活动组和对照组,TGF-β低于非活动组和对照组,SLE非活动组患者IL-6、IL-17、IL-23高于对照组。结论:SLE患者外周血Th17细胞与Treg细胞及相关细胞因子异常表达,并与疾病活动性相关,提示Th17/Treg比例失衡在SLE发病机制中发挥重要作用。测定Th17细胞和Treg细胞的表达,采用ELISA法检测白细胞介素-6(IL-6)、白细胞介素-17(IL-17)、白细胞介素-23(IL-23)、转化生长因子-β(TGF-β)的表达。结果:SLE活动组患者Ig A、Ig G、Ig M高于非活动组和对照组,C3、C4低于非活动组和对照组,SLE非活动组患者Ig A、Ig G高于对照组;SLE活动组患者Th17细胞、Th17/Treg高于非活动组和对照组,Treg细胞低于非活动组和对照组,SLE非活动组患者Th17细胞、Th17/Treg高于对照组,Treg细胞低于对照组;SLE活动组患者IL-6、IL-17、IL-23高于非活动组和对照组,TGF-β低于非活动组和对照组,SLE非活动组患者IL-6、IL-17、IL-23高于对照组。结论:SLE患者外周血Th17细胞与Treg细胞及相关细胞因子异常表达,并与疾病活动性相关,提示Th17/Treg比例失衡在SLE发病机制中发挥重要作用。

Objective： To explore the expressions and significance of Th17,Treg,and related cytokines in peripheral blood in patients with SLE. Methods： A total of 65 patients with SLE admitted in our hospital from May 2016 to April 2017 were included in the study and divided into the activity group（n = 33） and the non-activity group（n = 32） according to SLEDAI. Moreover,25 healthy individuals who came for physical examinations were served as the control group. The fasting peripheral venous blood was collected. The scattering velocity immunoturbidimetry was use to detect the serum Ig A,Ig G,Ig M,C3,C4 levels. FCM was used to detect the expressions of Th17 and Treg. ELISA was used to detect the expressions of IL-6,IL-17,IL-23,and TGF-β. Results： Ig A,Ig G,and Ig M in the activity group were significantly higher than those in the non-activity group and the control group,while C3 and C4 were significantly lower than those in the non-activity group and the control group. Ig A and Ig G in the non-activity group were significantly higher than those in the control group. Th17 and Th17/Treg in the activity group were significantly higher than those in the non-activity group and the control group,while Treg was significantly lower than that in the non-activity group and the control group. Th17 and Th17/Treg in the non-activity group were significantly higher than those in the control group,while Treg was significantly lower than that in the control group. IL-6,IL-17,and IL-23 in the activity group were significantly higher than those in the non-acitivity group and the control group,while TGF-βwas significantly lower than that in the non-acitivity group and the control group. IL-6,IL-17,and IL-23 in the non-activity group were significantly higher than those in the control group. Conclusion： The expressions of peripheral blood Th17,Treg,and related cytokines in patients with SLE are abnormally expressed,and correlated with the disease activity,sugessting that Th17/Treg imbalance plays a vital role in the pathogenesis of SLE.