PI3K抑制剂拮抗雌激素介导的子宫内膜癌细胞Ishikawa增殖的研究

The study of PI3K inhibitor antagonised the Estrogen-mediated proliferation of endometrial carcinoma cells Ishikawa

目的:探讨磷脂肌醇3-激酶(PI3K)抑制剂LY294002对雌激素(E2)介导下的子宫内膜癌Ishikawa细胞增殖和凋亡的影响。方法:CCK-8法检测E2对Ishikawa细胞的增殖作用,以及LY294002对Ishikawa细胞增殖的抑制作用;流式细胞仪检测Ishikawa细胞在E2与LY294002作用下的细胞凋亡情况;划痕实验检测细胞迁移能力。结果:E2促进Ishikawa细胞增殖,LY294002抑制E2介导下的Ishikawa细胞增殖,且呈时间浓度依赖性。LY294002作用下,Ishikawa细胞失去了迁移侵袭能力,细胞大量凋亡。结论:E2促进Ishikawa细胞的增殖,具有时间和浓度依赖性,LY294002对E2介导下的Ishikawa细胞增殖具有抑制作用。

Objective： To observe the influence of PI3 K inhibitor LY294002 on proliferation and apoptosis of endometrial carcinoma cells stimulated by E2. Methods： The effects of LY294002 on E2-induced proliferation and apoptosis were detected by CCK-8 assay and fluorescence-activated cell sorting technique. The migration ability of cells was examined by scratch test.Results： LY294002 decreased E2-induced proliferation in Ishikawa cells in a time-dependent and concentration dependent manner.Scratch test showed that Ishikawa cells lost the ability of migration and invasion with LY294002.LY294002 induced Ishikawa cells apoptosis. Conclusion： E2 have positive efforts on proliferation of Ishikawa cells in a time-dependent and concentration dependent manner.LY294002 can inhibit Ishikawa cells proliferation induced by E2.