两种低氧性肺动脉高压小鼠模型的建立与比较

Establishment and comparison of two hypoxic pulmonary hypertension models in mice

目的比较单纯低氧与低氧联合SU5416建模方法的异同,探索低氧联合SU5416能否建立更显著的肺动脉高压小鼠模型。方法 15只雄性C57BL/6小鼠采用数字表法随机分为3组(每组5只):常氧组、单纯低氧组和低氧联合SU5416组,4周后,检测各组小鼠体质量(body weight,BW)、右心室收缩压(right ventricular systolic pressure,RVSP)、右心室肥厚指数(the index of right ventricular hypertrophy,RVHI)、右心室/体质量比值(right ventricle/body weight,RV/BW),并检测其肺小动脉的形态学改变以及血管中膜厚度占血管外径百分比(medial thickness,MT%)。结果 4周后,常氧组小鼠的体质量明显高于单纯低氧组和低氧联合SU5416组,单纯低氧组、低氧联合SU5416组小鼠的RVSP、RVHI、RV/BW、MT%均高于常氧组,且低氧联合SU5416组小鼠的RVSP、RVHI、MT%三项指标较单纯低氧组更高,而低氧联合SU5416组小鼠的RV/BW与单纯低氧组差异无统计学意义。结论低氧联合SU5416能建立比单纯低氧更显著的肺动脉高压小鼠模型。

Objective To compare the similarities and differences between the model of hypoxia and hypoxia combined SU5416 modeling method,explore whether hypoxia combined with SU5416 can establish a more significant model of pulmonary hypertension in mice. Methods Totally 15 male C57 BL/6 mice were randomly divided into 3 groups： normoxia,hypoxia and hypoxia ＋ SU5416. After four weeks experiment,body weight（BW）,right ventricular systolic pressure（RVSP）,right ventricular hypertrophy index（RVHI）,right ventricular/body weight（RV/BW）,lung morphological features and medial thickness（MT%） were assessed. Results After 4 weeks,the body weight of the mice in normoxia was significantly higher than that in hypoxia and hypoxia ＋ SU5416,whereas the RVSP,RVHI,RV/BW and MT% of the mice in hypoxia and hypoxia ＋ SU5416 were higher than that in normoxia. The RVSP,RVHI and MT%of the hypoxia ＋ SU5416 were higher than those in hypoxia,while the RV/BW of hypoxia ＋ SU5416 was not significantly different from that of hypoxia. Conclusion These results suggested that hypoxia combined with SU5416 can establish a more significant pulmonary hypertension model than hypoxia alone in mice.